1. Spingophosphonolipids in microsomes of Diplodon delodontus. Distribution and effect on the membrane microviscosity
- Author
-
Carlos E. Irazú and Ricardo J. Pollero
- Subjects
chemistry.chemical_classification ,Ceramide ,Chromatography ,biology ,Physiology ,Diplodon ,Fatty acid ,General Medicine ,biology.organism_classification ,Biochemistry ,Sphingolipid ,Palmitic acid ,Microviscosity ,chemistry.chemical_compound ,Membrane ,chemistry ,Microsome ,lipids (amino acids, peptides, and proteins) ,Molecular Biology - Abstract
1. 1. The distribution of ceramide aminoethylphosphonate (CAEP) in microsomal membranes obtained from different tissues of the bivalve mollusc Diplodon delodontus was determined. 2. 2. The concentration of CAEP reached from 9 to 19% of the total microsomal polar lipids, depending on the kind of tissue. 3. 3. Palmitic acid was the main fatty acid in the ceramide moiety, followed by stearic and eicosamonoenoic acids. 4. 4. Artificial membranes were prepared with microsomal phospholipids or phospholipids plus sterols, with and without the addition of CAEP. 5. 5. It was shown that the phosphonate confers minor mobility to the membranes. This effect is more effective when the membrane contains the natural sterols and the phospholipids are unsaturated.
- Published
- 1990
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