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Your search keyword '"Sasazaki S"' showing total 2 results
Start Over Author "Sasazaki S" Journal comparative biochemistry and physiology part a molecular integrative physiology
2 results on '"Sasazaki S"'

1. Accumulation of caveolin-3 protein is limited in damaged muscle in chicken muscular dystrophy.

Author

Matsumoto H, Sasazaki S, Fujiwara A, Ichihara N, Kikuchi T, and Mannen H

Subjects
Animals, Chickens, Female, Male, Muscular Dystrophies pathology, Phenotype, Reverse Transcriptase Polymerase Chain Reaction, Caveolin 3 genetics, Caveolin 3 metabolism, Muscle, Skeletal metabolism, Muscle, Skeletal pathology, Muscular Dystrophies genetics, Muscular Dystrophies metabolism
Abstract

Members of the caveolin family are the main component of caveolae, and caveolin-3 is a muscle-specific protein. Caveolin-3 deficiency induces a muscular dystrophic phenotype, while its overexpression is also harmful to muscle cells. Increased caveolae were observed in chicken muscular dystrophy; however, the underlying mechanism causing the onset remains unclear. Therefore, the current study analyzes the expression of caveolin-3 and other caveola-related proteins in dystrophic chickens. Western blotting and semi-quantitative RT-PCR analysis revealed that (1) caveolin-3 is highly expressed in the damaged muscle of dystrophic chickens (7.12-fold); (2) the amount of caveolin-3 protein is regulated in posttranslational modification, since no significant increase is observed at the mRNA level (1.09-fold); and (3) the expression pattern of other caveola-related proteins is similar to that of caveolin-3. These results suggest that the accumulation of caveolin-3 protein may be associated with the causative process of chicken muscular dystrophy., (Copyright (c) 2010 Elsevier Inc. All rights reserved.)

Published
2010
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2. Stearoyl-CoA desaturase mRNA expression during bovine adipocyte differentiation in primary culture derived from Japanese Black and Holstein cattle.

Author

Ohsaki H, Sawa T, Sasazaki S, Kano K, Taniguchi M, Mukai F, and Mannen H

Subjects
Animals, CCAAT-Enhancer-Binding Protein-alpha genetics, CCAAT-Enhancer-Binding Protein-alpha metabolism, Cattle, Cells, Cultured, Enzyme Induction, Fatty Acid-Binding Proteins genetics, Fatty Acid-Binding Proteins metabolism, Fatty Acids metabolism, Male, Reverse Transcriptase Polymerase Chain Reaction, Stearoyl-CoA Desaturase genetics, Sterol Regulatory Element Binding Protein 1 genetics, Sterol Regulatory Element Binding Protein 1 metabolism, Time Factors, Adipocytes enzymology, Adipogenesis genetics, RNA, Messenger biosynthesis, Stearoyl-CoA Desaturase biosynthesis
Abstract

Stearoyl-CoA desaturase (SCD) catalyzes the synthesis of monounsaturated fatty acids (MUFAs). In cattle, the MUFAs are related to softness and flavor of meat. In order to investigate gene expression profile during bovine preadipocyte differentiation, we isolated stromal-vascular cells from perirenal adipose tissues of Japanese Black and Holstein steers. Gene expression level of adipocyte type fatty acid binding protein (FABP4), SCD, sterol regulatory element binding protein 1 (SREBP1) and CCAAT/enhancer binding protein alpha (C/EBP-alpha) were elucidated by real-time PCR assay. The levels of SCD mRNA expression were significantly increased to 10.8 and 6.3-fold in Japanese Black and Holstein, respectively, on day 1 of the culture. The difference in SCD expression between the two breeds may reflect differences in the fat development characteristics of the cattle breeds. Although transcription factors SREBP1 and C/EBP-alpha are supposed to regulate SCD expression, expression levels of the two factors were not completely consistent with that of SCD.

Published
2007
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