1. Tryptophan-functionalized gold nanoparticles for deep UV imaging of microbial cells
- Author
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Vladimir Djoković, Dušan K. Božanić, Slavka Kascakova, Suzana Dimitrijević-Branković, Radovan Dojčilović, Emanuela Piscopiello, Vesna Vodnik, Matthieu Réfrégiers, Aleksandar R. Milosavljević, Adriaan S. Luyt, Jelena Pajovic, and Piscopiello, E.
- Subjects
Metal Nanoparticles ,Fluorescence imaging ,Colloid and Surface Chemistry ,X-ray photoelectron spectroscopy ,Microscopy, Electron, Transmission ,Escherichia coli ,Organic chemistry ,Gold nanoparticles ,Physical and Theoretical Chemistry ,Fourier transform infrared spectroscopy ,Functionalization ,E. coli ,Tryptophan ,High-resolution transmission electron microscopy ,Indole test ,Chemistry ,Surfaces and Interfaces ,General Medicine ,Fluorescence ,Colloidal gold ,Surface modification ,Spectrophotometry, Ultraviolet ,Gold ,Biotechnology ,Nuclear chemistry - Abstract
Biocompatible fluorescent nanostructures were prepared by a functionalization of gold nanoparticles with the amino acid tryptophan. The gold-tryptophan bioconjugates were investigated by TEM and HRTEM and various spectroscopy methods (XPS, FTIR, UV-vis and photoluminescence). It was found that the gold nanoparticles, initially 8. nm in diameter, aggregate in the presence of the amino acid. From the XPS and FTIR spectroscopy results, it was concluded that the tryptophan gold interactions mainly take place via indole and carboxyl groups. Although the indole group is involved in the interaction with the gold surfaces, the tryptophan-gold hybrids showed strong fluorescence due to the presence of multilayers of tryptophan. Deep ultra violet (DUV) imaging performed at the SOLEIL synchrotron showed that it is possible to detect these hybrid nanostructures within Escherichia coli cells. Ministry of Education and Science of the Republic of Serbia (Project Nos. 172056, 45020, and 171020). Scopus
- Published
- 2015