1. The multilayer nanoparticles for deep penetration of docetaxel into tumor parenchyma to overcome tumor microenvironment.
- Author
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Khaliq NU, Park DY, Lee JY, Joo Y, Oh KS, Kim JS, Kim JS, Kim IS, Kwon IC, and Yuk SH
- Subjects
- Animals, Antibiotics, Antineoplastic administration & dosage, Antibiotics, Antineoplastic pharmacokinetics, Apoptosis drug effects, Cell Proliferation drug effects, Docetaxel, Drug Carriers chemistry, Humans, Lung Neoplasms enzymology, Lung Neoplasms pathology, Male, Mice, Mice, Inbred C3H, Nanoparticles chemistry, Taxoids administration & dosage, Taxoids pharmacokinetics, Tissue Distribution, Tumor Cells, Cultured, Xenograft Model Antitumor Assays, Antibiotics, Antineoplastic pharmacology, Lung Neoplasms drug therapy, Nanoparticles administration & dosage, Taxoids pharmacology, Tumor Microenvironment drug effects
- Abstract
Deep penetration of the anticancer drug, docetaxel (DTX), into tumor parenchyma was demonstrated to achieve improved chemotherapy. For this purpose, a multistage nanostructure was designed and characterized using the multilayer nanoparticles (NPs). The multilayer NPs had a core/shell structure. The core was composed of the DTX-loaded Pluronic NPs (diameter: 12nm) that were transferred into the inner side of vesicles to form the vesicle NPs. Förster resonance energy transfer (FRET) in the NPs was observed to verify the incorporation of the DTX-loaded Pluronic NPs into the inner side of the vesicles during the formation of the vesicle NPs. Subsequently, the vesicle NPs were stabilized through Pluronic-lipid bilayer interaction to form the multilayer NPs. To examine the morphology and size distribution of the multilayer NPs, transmittance electron microscopy and dynamic light scattering were used. In vitro release behavior and toxicity were observed to verify the functionality of the multilayer NPs as nanocarriers for cancer therapy. Multistage functionality was evaluated by cellular uptake and tissue distribution behaviors of the multilayer NPs. The biodistribution of the multilayer NPs and their antitumor efficacy were also observed to understand the role of multistage functionality for improved chemotherapy., (Copyright © 2016 Elsevier B.V. All rights reserved.)
- Published
- 2016
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