Search

Biocompatible nanogels were prepared using thiol modified hyaluronic acid and diacrylated pluronic F127 polymer. A simple Michael type addition reaction of activated thiol groups on acrylate moiety lead to the formation of these nanogels, which were further effectively fabricated with an anticancer drug for evaluating sustained drug release approach. Nanogels prepared were of 150 nm in diameter with a narrow size distribution pattern. DOX released from these nanogels showed a slow and sustained release at acidic pH 5.0 as compared to minimal release at physiological pH 7.4. Cytotoxicity data revealed the higher efficiency of DOX loaded nanogels as compared to free DOX in Hela cell lines. Cellular uptake images supported the cytotoxicity data and displayed DOX intercalation at nuclear level of cells. The sustained drug delivery system showed DOX release after 24 h and continued thereafter without affecting normal cells. Based on these findings, such nanogel system may be useful for delivering anticancer drug without hampering their toxicity value over longer durations and reducing the total dose amount in anticancer therapy. [ABSTRACT FROM AUTHOR]

[Display omitted] • Temperature and pH responsive microgels synthesized from amine modified Pluronic and EDTA using EDC coupling reaction. • Change in the ratio of EDTA affected the size and shape of the microgels. • Encapsulation of curcumin into the microgels enhanced its bioactivity and stability without altering its anticancer activity. • Temperature and pH played a significant role in the release of curcumin from the microgels and also in the anticancer activity. We developed here stimuli responsive curcumin loaded microgels based on Pluronic F-127. These microgels were prepared using coupling reaction between the amine modified Pluronic and EDTA. The microgel exhibited the affinity for hydrophobic drug, curcumin and showed pH as well as temperature-dependent release. Furthermore, the cytotoxicity study demonstrated dose-dependent inhibition of MDA-MB-231 cell growth with the most effective IC 50 value (3.8 ± 0.2 μg mL−1 after 24 h). Based on these findings, the fabricated curcumin loaded microgels offered additional advantages over conventional drug therapies for treatment of cancer. [ABSTRACT FROM AUTHOR]

• Fabrication of curcumin-loaded gelatin nanofibers using a non-toxic solvent • Nanofibers mats were prepared with varying the crosslinker percentage. • The rate of curcumin release was affected by the crosslinking density of fibers. • Fiber and mats displayed efficient cell migration in fibroblast and cancerous cell lines. • Promising anti-inflammatory and antibacterial activity for wound healing application. [ABSTRACT FROM AUTHOR]