1. Novel hydrogel comprising non-ionic copolymer with various concentrations of pharmacologically active bile acids for cellular injectable gel.
- Author
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Kovacevic, Bozica, Wagle, Susbin Raj, Ionescu, Corina Mihaela, Jones, Melissa, Lewkowicz, Michael, Wong, Elaine YM, Kojic, Sanja, Stojanovic, Goran, Đanić, Maja, Mikov, Momir, Mooranian, Armin, and Al-Salami, Hani
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BILE acids , *RHEOLOGY , *DEOXYCHOLIC acid , *HYDROGELS , *ANAEROBIC metabolism , *PANCREATIC beta cells - Abstract
Deoxycholic acid (DCA) is a bile acid capable of forming micelles and modifying the properties of hydrogels. We incorporated DCA in sodium alginate (SA) and poloxamer 407 matrices creating novel DCA-copolymer hydrogel for therapeutic delivery. Hydrogels were assessed for common rheological properties. Biocompatibility and biological effect were examined on various cell lines. Cell viability was determent in normal and various hypoxic conditions, and full mitochondrial bioenergetic parameters were assessed in cell lines in order to illustrate hydrogel effects on survival, and cell metabolic profile within the hydrogels. Obtained data suggest that a low dose of DCA in permeable, biocompatible hydrogels can be beneficial for cells to combat hypoxic conditions. [Display omitted] • Effects of hydrogels with deoxycholic acid were investigated on cells. • Hydrogels with bile acid may promote anaerobic metabolism in cells. • Hydrogels with bile acid may promote non-mitochondrial oxygen consumption in cells. • An increase in bile acid concentration leads to a decrease in beta cells' viability. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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