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26 results on '"Harland, Richard"'

21. Obtaining XenopusEggs and Embryos

Author

Sive, Hazel L. and Harland, Richard M.

Abstract

Collecting eggs from adult Xenopus laevisand Xenopus tropicalisto raise healthy embryos and tadpoles is relatively simple but requires careful handling of the frog. Eggs can be fertilized through natural matings or by in vitro fertilization and examined visually. Here we review how eggs are obtained and how to recognize healthy eggs that will develop into high-quality embryos.

Published
2023
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22. XenopusHusbandry

Author

Harland, Richard M. and L. Sive, Hazel

Abstract

Adult frogs that are well-cared-for will give high-quality eggs and embryos for use in every Xenopusprotocol. Thoughtful frog husbandry is thus pivotal to successful research using these organisms. Protocols for successfully raising tadpoles, establishing and maintaining water quality, and detecting specific pathogens are key to maintaining healthy frog populations.

Published
2023
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23. Obtaining Xenopus Eggs and Embryos.

Author

Sive HL and Harland RM

Subjects
Animals, Xenopus laevis, Xenopus, Larva, Fertilization, Fertilization in Vitro
Abstract

Collecting eggs from adult Xenopus laevis and Xenopus tropicalis to raise healthy embryos and tadpoles is relatively simple but requires careful handling of the frog. Eggs can be fertilized through natural matings or by in vitro fertilization and examined visually. Here we review how eggs are obtained and how to recognize healthy eggs that will develop into high-quality embryos., (© 2023 Cold Spring Harbor Laboratory Press.)

Published
2023
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24. Xenopus Husbandry.

Author

Harland RM and L Sive H

Subjects
Animals, Larva, Xenopus laevis
Abstract

Adult frogs that are well-cared-for will give high-quality eggs and embryos for use in every Xenopus protocol. Thoughtful frog husbandry is thus pivotal to successful research using these organisms. Protocols for successfully raising tadpoles, establishing and maintaining water quality, and detecting specific pathogens are key to maintaining healthy frog populations., (© 2023 Cold Spring Harbor Laboratory Press.)

Published
2023
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25. Isolation of DNA from red blood cells in Xenopus.

Author

Sive HL, Grainger RM, and Harland RM

Subjects
Animals, Molecular Biology methods, DNA isolation & purification, Erythrocytes, Xenopus laevis
Abstract

The amphibian Xenopus laevis is an important model organism that is particularly valuable for studies of early vertebrate development. Genomic DNA constitutes the total genetic information of an organism and it is used for Southern blotting, for determining gene structure, and for detecting the presence or absence of genes of interest. Genomic DNA can be extracted from Xenopus red blood cells, which are unlike the mammalian equivalent in that they contain nuclei. This article describes a protocol for the isolation of genomic DNA from frog red blood cells.

Published
2012
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26. Microinjection of RNA and preparation of secreted proteins from Xenopus oocytes.

Author

Sive HL, Grainger RM, and Harland RM

Subjects
Animals, Embryo, Nonmammalian, Recombinant Proteins genetics, Recombinant Proteins metabolism, Staining and Labeling methods, Sulfur Radioisotopes metabolism, Microinjections methods, Oocytes, RNA genetics, RNA metabolism, Recombinant Proteins isolation & purification, Xenopus
Abstract

Microinjection of Xenopus oocytes or embryos with messenger RNA (mRNA) is a powerful technique for studying development, including protein expression during development. This protocol describes a method for the preparation of secreted (35)S-labeled proteins following mRNA microinjection into Xenopus oocytes.

Published
2010
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