Search

Your search keyword '"Amanda Brosius Lutz"' showing total 2 results
Start Over Author "Amanda Brosius Lutz" Journal cold spring harbor protocols
2 results on '"Amanda Brosius Lutz"'

1. Purification of schwann cells from the neonatal and injured adult mouse peripheral nerve

Author

Amanda Brosius Lutz

Subjects
Pathology, medicine.medical_specialty, Aging, Primary culture, business.industry, Extramural, Dissection, Dissection (medical), Cell Separation, medicine.disease, Sciatic Nerve, General Biochemistry, Genetics and Molecular Biology, Mice, nervous system, Animals, Newborn, Peripheral nerve, Medicine, Animals, Trypsin, Sciatic nerve, Schwann Cells, business
Abstract

This protocol describes the use of immunopanning for acute purification and primary culture of Schwann cells from intact neonatal and injured adult mouse sciatic nerve.

Published
2014

2. Purification of Schwann Cells

Author

Amanda Brosius Lutz

Subjects
Pathology, medicine.medical_specialty, Primary culture, business.industry, Extramural, Cell Separation, Mouse Schwann Cell, Sciatic nerve injury, medicine.disease, General Biochemistry, Genetics and Molecular Biology, Mice, Animals, Newborn, nervous system, Peripheral nerve, Animals, Medicine, Schwann Cells, Sciatic nerve, business, Cells, Cultured
Abstract

This article introduces methods for the acute purification and primary culture of Schwann cells from the mouse sciatic nerve. Immunopanning can be used to isolate Schwann cells from intact nerves during early postnatal development as well as to purify Schwann cells from adult nerves following sciatic nerve injury. These methods facilitate the exploration of mouse Schwann cell biology in the healthy and injured peripheral nerve.

Published
2014

Catalog

Books, media, physical & digital resources
See catalog results