1. Association of vdr, cyp27b1, cyp24a1 and mthfr gene polymorphisms with oral lichen planus risk
- Author
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Zvonko Magic, Dragan Stanimirovic, Bojan Kujundzic, Barbara Jovanovic, Marko Magic, Vesna Ilic, Katarina Zeljic, and Gordana Supic
- Subjects
medicine.medical_specialty ,TaqI ,Genotype ,Single-nucleotide polymorphism ,Gastroenterology ,Calcitriol receptor ,Polymorphism, Single Nucleotide ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,stomatognathic system ,Gene Frequency ,CYP27B1 ,Internal medicine ,Medicine ,Humans ,Genetic Predisposition to Disease ,Vitamin D3 24-Hydroxylase ,General Dentistry ,VDR ,2. Zero hunger ,25-Hydroxyvitamin D3 1-alpha-Hydroxylase ,biology ,business.industry ,Haplotype ,030206 dentistry ,Single nucleotide polymorphisms ,medicine.disease ,FokI ,3. Good health ,stomatognathic diseases ,CYP24A1 ,chemistry ,030220 oncology & carcinogenesis ,Methylenetetrahydrofolate reductase ,Case-Control Studies ,Oral lichen planus ,MTHFR ,biology.protein ,Receptors, Calcitriol ,business ,Lichen Planus, Oral - Abstract
The current study investigated the association between VDR EcoRV (rs4516035), FokI (rs2228570), ApaI (rs7975232) and TaqI (rs731236), CYP27B1 (rs4646536), CYP24A1 (rs2296241), and MTHFR (rs1801133) gene polymorphisms and risk of oral lichen planus (OLP) occurrence. The study group consisted of 65 oral lichen planus patients and 100 healthy blood donors in the control group. Single nucleotide polymorphisms were genotyped by real time PCR or PCR-restriction fragment length polymorphism (RFLP) method. Heterozygous as well as mutated genotype of vitamin D receptor (VDR) FokI (rs2228570) polymorphism was associated with increased oral lichen planus risk in comparison with wild type genotype (odds ratio (OR) = 3.877, p = 0.017, OR = 38.153, p = 0.001, respectively). A significantly decreased OLP risk was observed for heterozygous genotype of rs2296241 polymorphism in CYP24A1 gene compared with the wild type form (OR = 0.314, p = 0.012). VDR gene polymorphisms ApaI and TaqI were in linkage disequilibrium (Dā = 0.71, r 2 = 0.22). Identified haplotype AT was associated with decreased OLP risk (OR = 0.592, p = 0.047). Our results highlight the possible important role of VDR FokI (rs2228570) and CYP24A1 rs2296241 gene polymorphisms for oral lichen planus susceptibility. Identification of new molecular biomarkers could potentially contribute to determination of individuals with OLP predisposition.
- Published
- 2015