1. Detection of interleukin-2 is not useful for distinguishing between latent and active tuberculosis in clinical practice: a prospective cohort study
- Author
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Francesca Sánchez, Ángel Domínguez-Castellano, Jesús Agüero-Balbin, Diego J. Pargada-Ferrer, N. Jové, Elvira Pérez-Escolano, F. Morandeira-Rego, Edu A. Struzka, X. Casas-Garcia, R. Agüero-Balbín, Mónica Ríos, Juan F. Rodríguez-Gutiérrez, A. Gonzalez-Cuevas, A. Coira-Nieto, R. Rabuñal, Fernando Alcaide, Abel Pallarés-Sanmartín, Joan Climent, Miguel Santin, Luis Anibarro, M.D. López-Prieto, Antón Penas-Truque, M. Trigo-Daporta, A. Martinez-Meñaca, María Dolores González-Ripoll Navarro, [Santin, M.] Bellvitge Univ Hosp IDIBELL, Infect Dis Serv, Barcelona 08907, Spain, [Santin, M.] Univ Barcelona, Dept Clin Sci, Barcelona, Spain, [Morandeira-Rego, F.] Bellvitge Univ Hosp IDIBELL, Serv Immunol, Barcelona, Spain, [Alcaide, F.] Bellvitge Univ Hosp IDIBELL, Microbiol Serv, Barcelona, Spain, [Alcaide, F.] Univ Barcelona, Dept Pathol & Expt Therapy, Barcelona, Spain, [Rabunal, R.] Hosp Univ Lucus Augusti, Infect Dis Unit, Lugo, Spain, [Anibarro, L.] Complexo Hosp Univ Pontevedra, Serv Infect Dis Internal Med, Unit TB, Pontevedra, Spain, [Aguero-Balbin, R.] Hosp Univ Marques de Valdecilla, Serv Resp Dis, Santander, Spain, [Martinez-Menaca, A.] Hosp Univ Marques de Valdecilla, Serv Resp Dis, Santander, Spain, [Casas-Garcia, X.] Hosp Gen Parc Sanitari St Joan de Deu, Resp Dis, Barcelona, Spain, [Perez-Escolano, E.] Hosp Jerez, Unidad Gest Clin Enfermedades Infecciosas & Micro, Cadiz, Spain, [Lopez-Prieto, M. D.] Hosp Jerez, Unidad Gest Clin Enfermedades Infecciosas & Micro, Cadiz, Spain, [Navarro, M. D.] Hosp Virgen Macarena, Clin Unit Infect Dis & Prevent Med, Seville, Spain, [Dominguez-Castellano, A.] Hosp Virgen Macarena, Clin Unit Infect Dis & Prevent Med, Seville, Spain, [Sanchez, F.] Hosp del Mar, IMIM, Infect Dis Serv, Barcelona, Spain, [Jove, N.] Hosp del Mar, IMIM, Infect Dis Serv, Barcelona, Spain, [Coira-Nieto, A.] Hosp Univ Lucus Augusti, Microbiol Serv, Lugo, Spain, [Trigo-Daporta, M.] Complexo Hosp Univ Pontevedra, Microbiol Serv, Pontevedra, Spain, [Gonzalez-Cuevas, A.] Hosp Gen Parc Sanitari St Joan de Deu, Microbiol Serv, Barcelona, Spain, and Ministerio de Economia y Competitividad, Instituto de Salud Carlos III (ISCIII) of the Spanish Government
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Adult ,Male ,0301 basic medicine ,Microbiology (medical) ,Interleukin 2 ,medicine.medical_specialty ,Tuberculosis ,Cells ,030106 microbiology ,QuantiFERON ,Interferon-gamma ,03 medical and health sciences ,0302 clinical medicine ,Latent Tuberculosis ,Memory ,Interquartile range ,Internal medicine ,medicine ,Humans ,Latent tuberculosis infection ,Interferon gamma ,Prospective Studies ,030212 general & internal medicine ,Prospective cohort study ,Tuberculosis, Pulmonary ,Incubation ,Aged ,Latent tuberculosis ,business.industry ,General Medicine ,Middle Aged ,bacterial infections and mycoses ,medicine.disease ,Active tuberculosis ,Infectious Diseases ,Spain ,Immunology ,Interleukin-2 ,Female ,business ,Biomarkers ,medicine.drug - Abstract
Previous reports have identified interleukin-2 (IL-2), quantified in the supernatants of QuantiFERONA multicentre study of 161 consecutive adult patients undergoing evaluation for active TB at eight TB Units in Spain. Interferon-γ (IFN-γ) and IL-2 were assessed in the supernatant of QFT after 16-24 h and 72 h of incubation. The accuracy of IL-2 for indicating latent TB infection (LTBI) was assessed by receiving operating characteristic curves. .Twenty-eight participants were not infected, 43 had LTBI, 69 had TB, and 21 were not classifiable. Median (interquartile range) IL-2 concentrations after 72 h of incubation were 0.0 pg/mL (0.0-0.0) in uninfected individuals, 261.0 pg/mL (81.0-853.0) in LTBI individuals, 166.5 pg/mL (33.5-551.5) in patients with extrapulmonary TB, 95.0 pg/mL (26.0-283.0) in patients with smear-negative pulmonary TB, and 38.5 pg/mL (7.5-178.0) in patients with smear-positive pulmonary TB (p0.0001). The area under the curve of the receiving operating characteristic curve (95% CI) of IL-2 after 72 h of incubation for the diagnosis of LTBI was 0.63 (0.53-0.74) when all TB cases were considered as a single group, ranging from 0.59 (0.47-0.71) to 0.72 (0.58-0.85) when only extrapulmonary and smear-positive pulmonary TB cases respectively were considered.Quantification of IL-2 in the supernatant of QFT after a prolonged incubation is not useful to distinguish between LTBI and active disease in clinical practice.
- Published
- 2016
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