1. Humoral immunity induced by mucosal and/or systemic SIV-specific vaccine platforms suggests novel combinatorial approaches for enhancing responses
- Author
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Steven G. Reed, Blake Frey, Jay A. Berzofsky, Viraj Kulkarni, Diego A. Vargas-Inchaustegui, Thorsten Demberg, L. Jean Patterson, George N. Pavlakis, Yongjun Sui, Marjorie Robert-Guroff, Antonio Valentin, Shari N. Gordon, Margherita Rosati, Iskra Tuero, Barbara K. Felber, Janet DiPasquale, Genoveffa Franchini, David C. Montefiori, Candido Alicea, Poonam Pegu, David Venzon, Thomas Musich, Niranjan Y. Sardesai, Yichuan Wang, Venkatramanan Mohanram, Guy R. Pilkington, Jenifer Bear, Namal P.M. Liyanage, and Alison Hogg
- Subjects
Enzyme-Linked Immunospot Assay ,Time Factors ,viruses ,Immunology ,Simian Acquired Immunodeficiency Syndrome ,Priming (immunology) ,Biology ,Antibodies, Viral ,medicine.disease_cause ,Article ,Immunoglobulin G ,Immune system ,Vaccines, DNA ,medicine ,Animals ,Humans ,Immunology and Allergy ,Immunity, Mucosal ,AIDS Vaccines ,Antibody-dependent cell-mediated cytotoxicity ,Vaccination ,Antibody-Dependent Cell Cytotoxicity ,SAIDS Vaccines ,Gene Products, env ,virus diseases ,Simian immunodeficiency virus ,Macaca mulatta ,Virology ,Immunoglobulin A ,Vaccines, Subunit ,Humoral immunity ,biology.protein ,Drug Therapy, Combination ,Simian Immunodeficiency Virus ,Antibody - Abstract
Combinatorial HIV/SIV vaccine approaches targeting multiple arms of the immune system might improve protective efficacy. We compared SIV-specific humoral immunity induced in rhesus macaques by five vaccine regimens. Systemic regimens included ALVAC-SIVenv priming and Env boosting (ALVAC/Env); DNA immunization; and DNA plus Env co-immunization (DNA&Env). RepAd/Env combined mucosal replication-competent Ad-env priming with systemic Env boosting. A Peptide/Env regimen, given solely intrarectally, included HIV/SIV peptides followed by MVA-env and Env boosts. Serum antibodies mediating neutralizing, phagocytic and ADCC activities were induced by ALVAC/Env, RepAd/Env and DNA&Env vaccines. Memory B cells and plasma cells were maintained in the bone marrow. RepAd/Env vaccination induced early SIV-specific IgA in rectal secretions before Env boosting, although mucosal IgA and IgG responses were readily detected at necropsy in ALVAC/Env, RepAd/Env, DNA&Env and DNA vaccinated animals. Our results suggest that combined RepAd priming with ALVAC/Env or DNA&Env regimen boosting might induce potent, functional, long-lasting systemic and mucosal SIV-specific antibodies.
- Published
- 2014