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Background: The use of dried blood spots on filter paper is well documented as an affordable and practical alternative to classical venous sampling for various clinical needs. This technique has indeed many advantages in terms of collection, biological safety, storage, and shipment. Amyloid β (A β ) peptides are useful cerebrospinal fluid (CSF) biomarkers for Alzheimer disease diagnosis. However, A β determination is hindered by preanalytical difficulties in terms of sample collection and stability in tubes. Methods: We compared the quantification of A β peptides (1 – 40, 1 – 42, and 1 – 38) by simplex and multiplex ELISA, following either a standard operator method (liquid direct quantification) or after spotting CSF onto dried matrix paper card. Results: The use of dried matrix spot (DMS) overcame preanalytical problems and allowed the determination of A β concentrations that were highly commutable (BlandAltman) with those obtained using CSF in classical tubes. Moreover, we found a positive and significant correlation (r 2 = 0.83, Pearson coefficient p = 0.0329) between the two approaches. Conclusions: This new DMS method for CSF represents an interesting alternative that increases the quality and efficiency in preanalytics. This should enable the better exploitation of A β analytes for Alzheimer ’ s diagnosis.