Search

A rapid method for the estimation of urinary 17-ketosteroids, based on the Zimmerman reaction, is presented. The color complex due to ketosteroids is selectively extracted with chloroform. Turbidity of chloroform extract is avoided by the addition of polyethyleneglycol.

Strips of filter paper are impregnated with a buffered acetylcholine substrate solution, containing phenol red as an indicator, and 1,2-propyleneglycol serving as an inhibitor of bacterial decomposition and as a wetting agent. The test is suitable for the detection of the poison in vivo, as well as in suspected materials such as food, clothing, water, soil, and air samples. The test is based on the inhibition of acetylcholinesterase by the organophosphoric group of the substances. In positive tests the indicator paper turns pink-to-violet and in negative tests it turns a yellow color.