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1. Unequal representation of different unique genomic DNA sequences in the cell-free plasma DNA of individual donors.

Author

Puszyk WM, Crea F, and Old RW

Subjects
Base Sequence, Humans, Polymerase Chain Reaction, Sequence Analysis, DNA, DNA blood, DNA chemistry
Abstract

Objectives: To assess whether different genomic cell-free DNAs are equally abundant in the plasma of individual donors, and any relationship between DNA methylation and representation in plasma., Design and Methods: The concentrations of DNA in plasma were determined by real-time PCR., Results: Different DNA sequences were not equally represented. The relative abundances were similar in different donors., Conclusions: Different DNA sequences are not equally abundant in plasma, with no relationship between DNA methylation and abundance.

Published
2009
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