1. HLA-DR(hi) and CCR9 Define a Pro-Inflammatory Monocyte Subset in IBD
- Author
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Jeanette Grundström, Michael Eberhardson, Emma Lindh, Izabella Janczewska, Annelie Lindberg, Hans Glise, Per Karlén, Eric Hjalmarsson, Ola Winqvist, Ludvig Linton, Ragnar Befrits, and Mats Karlsson
- Subjects
business.industry ,Inflammatory Bowel Disease ,Gastroenterology ,CCR9 ,Inflammatory monocyte ,Human leukocyte antigen ,medicine.disease ,Bioinformatics ,Inflammatory bowel disease ,Phenotype ,digestive system diseases ,Intestinal mucosa ,Intestinal inflammation ,Immunology ,medicine ,In patient ,business - Abstract
OBJECTIVES: It has been demonstrated that circulating monocytes relocate to the intestinal mucosa during intestinal inflammation, but the phenotype and inflammatory mechanisms of these monocytes remain poorly understood. Here, we have investigated blood monocytes expressing high levels of HLA-DR and CCR9 in patients with inflammatory bowel disease (IBD). METHODS: Fifty-one patients with mild to severe ulcerative colitis (UC; n=31; UC-DAI 3–12) or Crohn's disease (CD; n=20; Harvey–Bradshaw indices (HBI) 2–16) were included together with 14 controls, during IBD therapy for four consecutive weeks. The frequency of CD14+HLA-DRhi monocytes was monitored weekly in peripheral blood, using flow cytometry. The surface phenotype and cytokine profile of these monocytes were established using flow cytometry and real-time PCR. Clinical parameters were assessed weekly in all patients. RESULTS: The frequency of circulating CD14+HLA-DRhi monocytes was significantly higher in IBD patients with moderate to severe disease compared with healthy controls (P
- Published
- 2012