1. Development of a novel Ultra Performance Liquid Chromatography Tandem-Mass Spectrometry (UPLC-MS/MS) method to measure l-arginine metabolites in plasma.
- Author
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Santucci, Lavinia, Lomuscio, Sara, Primiano, Aniello, Calvani, Riccardo, Persichilli, Silvia, Iavarone, Federica, Picca, Anna, Canu, Francesca, Urbani, Andrea, and Gervasoni, Jacopo
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CITRULLINE , *ASYMMETRIC dimethylarginine , *HYDROPHILIC interaction liquid chromatography , *LIQUID chromatography , *ARGININE , *SMALL molecules , *METABOLITES - Abstract
• Arginine metabolism is important to evaluate organism oxidative stress. • LC-MS/MS methods are considered gold standard for small molecules determination. • Developed method is easy to implement in research and routine purpose. Arginine metabolism is involved in the regulation of several biological processes. Many liquid chromatography tandem-mass spectrometry methods for the determination of arginine and its metabolites have been developed but they are time consuming and imply long pre-analytical procedures. The purpose of this study was to develop a rapid method for the simultaneous determination of arginine, citrulline, ornithine, symmetric and asymmetric dimethylarginine and monomethylarginine in human plasma. The pre-analytical procedure consisted in a simple deproteinization. The chromatographic separation was performed using hydrophilic interaction liquid chromatography. Analytes detection was performed with a triple quadrupole equipped with electrospray ion source operating in positive ion mode. Mass spectrometry experiments were conducted in multiple reaction monitoring mode. Recovery ranged from 92.2 to 108.0%. The within-run imprecision and between-run imprecision ranged from 1.5 to 6.8 % and 3.8 to 11.9%, respectively. Carry over and matrix effect did not affect quantitative analysis. Extraction recovery was between 95 and 105 %. Stability after pre-analytical procedure was tested and all the metabolites were stable after 48 h at 4 °C. In conclusion, our novel method allow a rapid and easy determination of arginine and its metabolites both for research and clinical routine use. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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