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This study aimed to analyze the phytochemical profile and drying with prediction of the leaves of Ocimum citriodurum L, quantify the total phenolics and flavonoids and determine the unprecedented potential of the fungicidal activity of the oil-in-water nanoemulsion incorporated with essential oil and hydroalcoholic extract of O. citriodurum L. The leaves were carefully selected in the rural area of São Luís, Maranhão, Brazil, and subsequently crushed and stored. The essential oil was obtained by hydrodistillation (100°C / 3h). To obtain the hydroalcoholic extract, maceration in solvent extract ethanol PA 70% (v / v) was used. To determine the antifungal activity, the technique recommended by the International Clinical Laboratory Standard was used through the technique of Dilution in Broth and Sowing and Agar to determine the Minimum Inhibitory Concentration (MIC) and Minimum Fungicidal Concentration (CFM), respectively, front to strains of Aspergillus niger (ATCC 6275), Colletotrichum gloeosporioides (ATCC 96723), Penicillium chrysogenum (ATCC 10106). The results obtained for fungicidal action attested to CIM and strong inhibition for the action of essential oils and hydroalcoholic extracts with a concentration of 250 µg mL-1 in front of A. niger, C. gloeosporioides and P. chrysogenum. CFM's of 300, 250 and 500 mL-1 were observed in front of A. niger, C. gloeosporioides and P. chrysogenum, respectively. With the results obtained it is stated that a stable nanoemulsion was obtained for both (EX) and (OE) and that they present active compounds in which they favor an excellent antifungal activity.

This work aimed to evaluate the fungicidal activity of nanoemulsions of hydroalcoholic extract and essential oil of sprouts of Syzygium aromaticum . For the extraction of essential oil, the hydrodistillation technique was used. To obtain the hydroalcoholic extract, the process of maceration with solvent extract methanol P.A 70% (v / v) was performed. Nanoemulsions were obtained using the low-energy phase inversion method. For the fungicidal activity, the Broth Dilution and Agar Seeding assay was used to determine the Minimum Inhibitory Concentration (MIC) and Minimum Fungicidal Concentration (CFM). The action of the biotechnological product was tested against three strains of fungi Aspergillus niger, Colletotrichum gloeosporioides, Penicillium chrysogenum . For antifungal action of the essential oil nanoemulsion, the MIC obtained was 25 µg mL -1 , 25 µg mL -1 and 50 µg mL -1 against A. niger, C. gloeosporioides and P. chrysogenum , respectively, for the hydroalcoholic extract nanoemulsion the result was 50 µg mL -1 , 50 µg mL -1 and 100 µg mL -1 for A. niger, C. gloeosporioides and P. chrysogenum , respectively. Finally, this study points to the nanoemulsion of S. aromaticum as an alternative fungicide in the control and combat of pathogenic fungi .

The increase in strains resistant to conventional antifungals means that there is a need for studies related to alternative therapeutic practices, such as medicinal plants. This study aims to evaluate the chemical profile and fungicidal activity of the nanoemulsion of the essential oil obtained from Ba-har (Syrian pepper). The phytochemical profile of the plant material was performed based on the methodology of Matos (2009). For extraction of essential oil, 100g of plant material was used, with the isolation of essential oil by the hydrodistillation technique conducted at 100 °C / 3h. To quantify the total phenolics present in the OE, the Folin-Ciocalteu method was used. For the total flavonoids, the AlCl 3 complexation method was used. The oil-in-water nanoemulsion was formulated by the low-energy method of phase inversion using essential oil, non-ionic surfactant and water, and the obtained nanoemulsion was subjected to thermodynamic stability tests. The essential oils and stable nanoemulsions were subjected to evaluation of the fungicidal action against strains of Aspergillus niger (ATCC 6275), Colletotrichum gloeosporioides (ATCC 96723) and Penicilium chrysogenum (ATCC 10106). The fungicidal activity was performed according to CLSI (2020) using the Broth Dilution method to obtain the Minimum Inhibitory Concentration (MIC) and sowing on agar for Minimum Fungicidal Concentration (CFM). The results obtained for the total phenolic content were quantified at 348.3 mg EAT g -1 and 346.21 mg EQ g -1 for flavonoids. Ba-har essential oil was more efficient in inhibiting A. niger , as it presented the lowest MIC (200 µg mL -1 ), followed by P. chrysogenum (250 µg mL -1 ) and later by C. gloeosporioides (300 µg mL -1 ). The identification of the secondary metabolites present in Ba-har was quite considerable, since they are responsible for the biological properties, thus inspiring the continuity of studies related to its biological activities. As for the total phenolic content and flavonoids present in the essential oil, they indicated the important antioxidant potential. The fungicidal potential of Ba-har oil showed strong inhibition and mortality, however the nanoemulsion product with the essential oil incorporated showed a more efficient action against the pathogenic fungi tested