47 results on '"Xin, Yi"'
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2. Change of serum B-cell activating factor level in patients with positive antiphospholipid antibodies and previous adverse pregnancy outcomes and its significance
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Xin-Yi Li, Hong-Ji Duan, Xiang-Yuan Liu, Xiao-Li Deng, and Peng Lyu
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Medicine - Abstract
Abstract. Background:. B-cell activating factor (BAFF) is vital for B cell survival. Serum BAFF levels are elevated in thrombotic antiphospholipid syndrome, but little is known about levels in patients with positive antiphospholipid antibodies (aPLs) and previous adverse pregnancy outcomes (APOs). We aimed to analyze serum BAFF concentrations of these patients in early pregnancy along with different pregnancy outcomes. Methods:. Thirty-six pregnant patients positive for aPLs and previous APOs (patient group), 25 healthy pregnant females (HP group) and 35 healthy non-pregnant females (HNP group) from the Peking University Third Hospital, between October 2018 and March 2019, were enrolled in this study. Serum of HNP and serum of patients as well as HP in the first gestational trimester were collected. Enzyme-linked immunosorbent assay kits were used to measure serum BAFF and interferon-alpha (IFN-α) concentrations. Cytometric bead array analysis was used to measure serum concentrations of cytokines. The patient group was further divided into APOs and non-APOs (NAPOs) group, fetal loss and live birth group according to pregnancy outcomes. The Mann-Whitney U-test was used to assess significance between and within groups. Spearman rank-order was used to evaluate correlation coefficients between BAFF and related cytokines. Results:. The serum BAFF level in HP group was significantly lower than HNP group (245.24 [218.80, 265.90] vs. 326.94 [267.31, 414.80] pg/mL, Z = −3.966, P
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- 2020
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3. Chemokine-like factor-like MARVEL transmembrane domain-containing family in autoimmune diseases
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Hong-Ji Duan, Xin-Yi Li, Chang Liu, Xiao-Li Deng, and Li-Shao Guo
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Medicine - Abstract
Abstract. The chemokine-like factor (CKLF)-like MARVEL transmembrane domain-containing family (CMTM) is widely expressed in the immune system. Abnormal expression of CMTM is associated with the development of various diseases. This article summarizes the relevant research on the role of the CMTM family in immune disorders. This information will increase our understanding of pathogenesis and identify promising targets for the diagnosis and treatment of autoimmune diseases. The CMTM family is highly expressed in peripheral blood mononuclear cells. CKLF1 may be involved in the development of arthritis through its interaction with C-C chemokine receptor 4. CKLF1 is associated with the pathogenesis of lupus nephritis and psoriasis. Both CMTM4 and CMTM5 are associated with the pathogenesis of systemic lupus erythematosus. CMTM1, CMTM2, CMTM3, and CMTM6 play a role in rheumatoid arthritis, systemic sclerosis, Sjögren syndrome, and anti-phospholipid syndrome, respectively. The CMTM family has been implicated in various autoimmune diseases. Further research on the mechanism of the action of CMTM family members may lead to the development of new treatment strategies for autoimmune diseases.
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- 2020
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4. Rhabdomyolysis and respiratory insufficiency due to the common ETFDH mutation of c.250G>A in two patients with late-onset multiple acyl-CoA dehydrogenase deficiency
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Hai-Zhu Chen, Ming Jin, Nai-Qing Cai, Xiao-Dan Lin, Xin-Yi Liu, Liu-Qing Xu, Min-Ting Lin, Feng Lin, Ning Wang, Zhi-Qiang Wang, Guo-Rong Xu, and Yi Cui
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Medicine - Published
- 2019
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5. Pharmacokinetic Studies of Factor VIII in Chinese Boys with Severe Hemophilia A: A Single-Center Study
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Zhen-Ping Chen, Pei-Jing Li, Gang Li, Ling Tang, Ying-Zi Zhen, Xin-Yi Wu, Xiao-Ling Cheng, Koon Hung Luke, Victor S Blanchette, Man-Chiu Poon, Qiu-Lan Ding, and Run-Hui Wu
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Boys ,Factor VIII ,Hemophilia A ,Pharmacokinetics ,Medicine - Abstract
Background: Although much attention has been paid to the pharmacokinetics (PKs) of different factor VIII (FVIII) concentrates in persons with hemophilia A (HA), limited information is available in young boys with severe HA. In this study, we aimed to assess the PK parameters of FVIII products in boys with severe HA in China. Methods: A total of 36 boys (plasma-derived [pd]-FVIII, n = 15; recombinant [r] FVIII, n = 21) were enrolled between January 2015 and May 2016 in Beijing Children's Hospital. PK characteristics of FVIII products were studied according to a reduced 4-sampling time point design (1 h, 9 h, 24 h, and 48 h postinfusion). Results: The mean FVIII half-life (t1/2) was 10.99 ± 3.45 h (range 5.52–20.02 h), the mean in vivo recovery (IVR) was 2.01 ± 0.42 IU/dl per IU/kg (range 1.24–3.02 IU/dl per IU/kg) and mean clearance (CL) of FVIII is 4.34 ± 1.58 ml·kg−1·h−1 (range 2.29–7.90 ml·kg−1·h−1). We also analyzed the influence of several parameters that potentially modulate FVIII PK. The age was closely associated with FVIII half-life (R2 = 0.32, P < 0.01). The t1/2of FVIII increased by 0.59 h per year. Besides age, von Willebrand factor antigen (VWF:Ag) also was associated with FVIII half-life (R2 = 0.52, P < 0.01). Patients with blood Group O had a shorter FVIII half-life than patients with non-O blood group (9.40 ± 0.68 h vs. 12.3 ± 0.79 h, t = 2.70, P = 0.01). The FVIII IVR correlated with age (R2 = 0.21, P < 0.01) and VWF:Ag level (R2 = 0.28, P < 0.01). CL rates were faster in young patients and in those with low-VWF:Ag levels. CL rates of FVIII are higher in blood Group O versus non-blood Group O persons (5.02 ± 0.38 vs. 4.00 ± 0.32 ml·kg−1·h−1, t = 2.53, P = 0.02). Conclusions: Chinese boys with severe HA have similar PK values to other ethnic groups and large differences in FVIII PK between individual patients. Age, blood group, and VWF:Ag levels are important determining factors for FVIII CL.
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- 2018
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6. Effect of Phosphorylated-Extracellular Regulated Kinase 1/2 Inhibitor on Retina from Light-induced Photoreceptor Degeneration
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Xin-Yi Ding, Rui-Ping Gu, Wen-Yi Tang, Qin-Meng Shu, Ge-Zhi Xu, and Meng Zhang
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Apoptosis ,Inflammation ,Retinal Degeneration ,Medicine - Abstract
Background: The demonstrated role of mitogen-activated protein kinase (MAPK) in both cell apoptosis and the inflammation pathway makes it an attractive target for photoreceptor protection. The aim of this study was to investigate the protective effects of MAPK antagonists against photoreceptor degeneration and retinal inflammation in a rat model of light-induced retinal degeneration. Methods: Sprague Dawley rats were treated with intravitreal injections of MAPK antagonists, inhibitors of p-P38, phosphorylated-extracellular regulated kinase (p-ERK) 1/2, and p-c-Jun N-terminal kinase (JNK) just before they were assigned to dark adaptation. After dark adaptation for 24 h, rats were exposed to blue light (2500 lux) in a light box for 24 h, and then returned to the normal 12-h light/12-h dark cycle. Samples were collected at different time points. MAPK expression during light exposure was examined with immunofluorescence. Photoreceptor death was detected with histopathology and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining. The expression of retinal p-ERK1/2, caspase 3, activated caspase 3, tumor necrosis factor (TNF)-α, and interleukin (IL)-1β was examined by Western blotting. Differences between groups were evaluated using unpaired one-way analysis of variance and least significant difference post hoc tests. Results: MAPKs (P38, ERK1/2, and p-JNK) were phosphorylated and activated in the light injury groups, compared with normal group, and their expressions were mainly elevated in the outer nuclear layer (ONL). Among the selected MAPK antagonists, only the p-ERK1/2 inhibitor attenuated the loss of photoreceptors and the thinning of ONL in light injury groups. Besides, p-ERK1/2 inhibitor refrained light-induced photoreceptor apoptosis, which was presented by TUNEL positive cells. Light injury significantly increased the expression of p-ERK1/2 (1.12 ± 0.06 vs. 0.57 ± 0.08, t = 9.99, P < 0.05; 1.23 ± 0.03 vs. 0.57 ± 0.08, t = 11.90, P < 0.05; and 1.12 ± 0.12 vs. 0.57 ± 0.08, t = 9.86, P < 0.05; F = 49.55, P < 0.001), and induced caspase 3 activating (0.63 ± 0.06 vs. 0.14 ± 0.05, t = 13.67, P < 0.05; 0.74 ± 0.05 vs. 0.14 ± 0.05, t = 16.87, P < 0.05; and 0.80 ± 0.05 vs. 0.14 ± 0.05, t = 18.57, P < 0.05; F = 100.15, P < 0.001), compared with normal group. The p-ERK1/2 inhibitor significantly reduced p-ERK1/2 overexpression (0.61 ± 0.06 vs. 1.12 ± 0.06, t = −9.26, P < 0.05; 0.77 ± 0.06 vs. 1.23 ± 0.03, t = −8.29, P < 0.05; and 0.68 ± 0.03 vs. 1.12 ± 0.12, t = −7.83, P < 0.05; F = 49.55, P < 0.001) and downregulated caspase 3 activating (0.23 ± 0.04 vs. 0.63 ± 0.06, t = −11.24, P < 0.05; 0.43 ± 0.03 vs. 0.74 ± 0.05, t = −8.86, P < 0.05; and 0.58 ± 0.03 vs. 0.80 ± 0.05, t = −6.17, P < 0.05; F = 100.15, P < 0.001), compared with light injury group. No significant change in the total level of caspase 3 was seen in different groups (F = 0.56, P = 0.75). As for inflammation, light injury significantly increased the expression of TNF-α (0.42 ± 0.04 vs. 0.25 ± 0.05, t = 5.99, P < 0.05; 0.65 ± 0.03 vs. 0.25 ± 0.05, t = 14.87, P < 0.05; and 0.86 ± 0.04 vs. 0.25 ± 0.05, t = 22.58, P < 0.05; F = 160.27, P < 0.001) and IL-1β (0.24 ± 0.01 vs. 0.19 ± 0.02, t = 2.33, P < 0.05; 0.35 ± 0.02 vs. 0.19 ± 0.02, t = 7.97, P < 0.05; and 0.48 ± 0.04 vs. 0.19 ± 0.02, t = 14.69, P < 0.05; F = 77.29, P < 0.001), compared with normal group. P-ERK1/2 inhibitor significantly decreased the overexpression of TNF-α (0.22 ± 0.02 vs. 0.42 ± 0.04, t = −7.40, P < 0.05; 0.27 ± 0.02 vs. 0.65 ± 0.03, t = −14.27, P < 0.05; and 0.33 ± 0.03 vs. 0.86 ± 0.04, t = −19.58, P < 0.05; F = 160.27, P < 0.001) and IL-1β (0.13 ± 0.03 vs. 0.24 ± 0.01, t = −5.77, P < 0.05; 0.17 ± 0.01 vs. 0.22 ± 0.02, t = −9.18, P < 0.05; and 0.76 ± 0.05 vs. 0.48 ± 0.04, t = −13.12, P < 0.05; F = 77.29, P < 0.001), compared with light injury group. Conclusion: The p-ERK1/2 inhibitor might protect the retina from light-induced photoreceptor degeneration and retinal inflammation.
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- 2018
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7. Skeletal Muscle Magnetic Resonance Imaging of the Lower Limbs in Late-onset Lipid Storage Myopathy with Electron Transfer Flavoprotein Dehydrogenase Gene Mutations
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Xin-Yi Liu, Ming Jin, Zhi-Qiang Wang, Dan-Ni Wang, Jun-Jie He, Min-Ting Lin, Hong-Xia Fu, and Ning Wang
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Electron Transfer Flavoprotein Dehydrogenase ,Fat Infiltration Atrophy ,Late-onset Lipid Storage Myopathy ,Magnetic Resonance Imaging ,Multiple Acyl-coenzyme A Dehydrogenation Deficiency ,Medicine - Abstract
Background: Lipid storage myopathy (LSM) is a genetically heterogeneous group with variable clinical phenotypes. Late-onset multiple acyl-coenzyme A dehydrogenation deficiency (MADD) is a rather common form of LSM in China. Diagnosis and clinical management of it remain challenging, especially without robust muscle biopsy result and genetic detection. As the noninvasion and convenience, muscle magnetic resonance imaging (MRI) is a helpful assistant, diagnostic tool for neuromuscular disorders. However, the disease-specific MRI patterns of muscle involved and its diagnostic value in late-onset MADD have not been systematic analyzed. Methods: We assessed the MRI pattern and fat infiltration degree of the lower limb muscles in 28 late-onset MADD patients, combined with detailed clinical features and gene spectrum. Fat infiltration degree of the thigh muscle was scored while that of gluteus was described as obvious or not. Associated muscular atrophy was defined as obvious muscle bulk reduction. Results: The mean scores were significantly different among the anterior, medial, and posterior thigh muscle groups. The mean of fat infiltration scores on posterior thigh muscle group was significantly higher than either anterior or medial thigh muscle group (P < 0.001). Moreover, the mean score on medial thigh muscle group was significantly higher than that of anterior thigh muscle group (P < 0.01). About half of the patients displayed fat infiltration and atrophy in gluteus muscles. Of 28 patients, 12 exhibited atrophy in medial and/or posterior thigh muscle groups, especially in posterior thigh muscle group. Muscle edema pattern was not found in all the patients. Conclusions: Late-onset MADD patients show a typical muscular imaging pattern of fat infiltration and atrophy on anterior, posterior, and medial thigh muscle groups, with major involvement of posterior thigh muscle group and gluteus muscles and a sparing involvement of anterior thigh compartment. Our findings also suggest that muscle MRI of lower limbs is a helpful tool in guiding clinical evaluation on late-onset MADD.
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- 2016
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8. A Historical Cohort Study on the Efficacy of Glucocorticoids and Riboflavin Among Patients with Late-onset Multiple Acyl-CoA Dehydrogenase Deficiency
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Xin-Yi Liu, Zhi-Qiang Wang, Dan-Ni Wang, Min-Ting Lin, and Ning Wang
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Glucocorticoids ,Historical Cohort Study ,Late-onset Multiple Acyl-CoA Dehydrogenase Deficiency ,Lipid Storage Myopathy ,Riboflavin ,Medicine - Abstract
Background: Late-onset multiple acyl-CoA dehydrogenase deficiency (MADD) is the most common type of lipid storage myopathies in China. Most patients with late-onset MADD are well responsive to riboflavin. Up to now, these patients are often treated with glucocorticoids as the first-line drug because they are misdiagnosed as polymyositis without muscle biopsy or gene analysis. Although glucocorticoids seem to improve the fatty acid metabolism of late-onset MADD, the objective evaluation of their rationalization on this disorder and comparison with riboflavin treatment are unknown. Methods: We performed a historical cohort study on the efficacy of the two drugs among 45 patients with late-onset MADD, who were divided into glucocorticoids group and riboflavin group. Detailed clinical information of baseline and 1-month follow-up were collected. Results: After 1-month treatment, a dramatic improvement of muscle strength was found in riboflavin group (P < 0.05). There was no significant difference in muscle enzymes between the two groups. Significantly, the number of patients with full recovery in glucocorticoids group was less than the number in riboflavin group (P < 0.05). On the other hand, almost half of the patients in riboflavin group still presented high-level muscle enzymes and weak muscle strength after 1-month riboflavin treatment, meaning that 1-month treatment duration maybe insufficient and patients should keep on riboflavin supplement for a longer time. Conclusions: Our results provide credible evidences that the overall efficacy of riboflavin is superior to glucocorticoids, and a longer duration of riboflavin treatment is necessary for patients with late-onset MADD.
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- 2016
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9. Pediatric Infectious Endophthalmitis: A 271-case Retrospective Study at a Single Center in China
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Meng Zhang, Ge-Zhi Xu, Rui Jiang, Ying-Qin Ni, Ke-Yan Wang, Rui-Ping Gu, and Xin-Yi Ding
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Endophthalmitis ,Ocular Trauma ,Pediatric ,Retina ,Medicine - Abstract
Background: Pediatric infectious endophthalmitis is a serious sight-threatening disease for children. The purpose of this study was to investigate the etiology, microbiological spectrum, and visual outcomes of infectious endophthalmitis in children at a single institution in China. Methods: It is a retrospective study of the medical records of all patients under 14 years of age with histories of infectious endophthalmitis, treated at a single institution from January 1, 2009 to January 1, 2015. The clinical characteristics, etiology, microbiological spectrum, and management, as well as the visual outcomes, were analyzed. The Kappa test and Chi-square test were used in the statistical evaluation. Results: A total of 271 children were identified, with a mean age of 5.61 ± 2.93 years (range 5 months to 14 years). Ocular trauma (94.8%) and previous ocular surgery (3.0%) were the most common etiologies. Overall, 147 (54.2%) cases had positive cultures, and 176 organisms were isolated from these patients. A single species was isolated in 120 (81.6%) cases, with multiple organisms in 27 (18.4%) cases, and the most commonly identified organisms were coagulase-negative Staphylococcus and Streptococcus species, comprising 29.5% and 26.8% of the isolates, respectively. Moreover, of 176 isolates, 142 (80.8%) were Gram-positive organisms, 23 (13.0%) were Gram-negative organisms, and 11 (6.2%) were fungi. The final visual outcomes were 20/200 or better in 66 (24.4%) eyes, counting fingers to 20/200 in 34 (12.5%), hand motions in 30 (11.1%), light perception in 33 (12.2%), no light perception in 32 (11.8%), and 9 (3.3%) eyes were enucleated or eviscerated. The visual outcomes were not available in 67 (24.7%) patients. Conclusions: Penetrating ocular trauma is the most frequent cause of pediatric endophthalmitis in China. Streptococcus and Staphylococcus species are the most commonly identified organisms in exogenous pediatric endophthalmitis whereas Fusarium species are commonly seen in endogenous endophthalmitis. In this research, in spite of aggressive management with antibiotics and vitrectomy, the visual prognosis was found to be generally poor.
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- 2016
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10. Targeted Genes Sequencing Identified a Novel 15 bp Deletion on GJA8 in a Chinese Family with Autosomal Dominant Congenital Cataracts
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Han-Yi Min, Peng-Peng Qiao, Asan, Zhi-Hui Yan, Hui-Feng Jiang, Ya-Ping Zhu, Hui-Qian Du, Qin Li, Jia-Wei Wang, Jie Zhang, Jun Sun, Xin Yi, and Ling Yang
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Congenital Cataract ,GJA8 ,Next Generation Sequencing ,Novel In-frame Deletion ,Targeted Genes Capture ,Medicine - Abstract
Background: Congenital cataract (CC) is the leading cause of visual impairment or blindness in children worldwide. Because of highly genetic and clinical heterogeneity, a molecular diagnosis of the lens disease remains a challenge. Methods: In this study, we tested a three-generation Chinese family with autosomal dominant CCs by targeted sequencing of 45 CC genes on next generation sequencing and evaluated the pathogenicity of the detected mutation by protein structure, pedigree validation, and molecular dynamics (MD) simulation. Results: A novel 15 bp deletion on GJA8 (c.426_440delGCTGGAGGGGACCCT or p. 143_147delLEGTL) was detected in the family. The deletion, concerned with an in-frame deletion of 5 amino acid residues in a highly evolutionarily conserved region within the cytoplasmic loop domain of the gap junction channel protein connexin 50 (Cx50), was in full cosegregation with the cataract phenotypes in the family but not found in 1100 control exomes. MD simulation revealed that the introduction of the deletion destabilized the Cx50 gap junction channel, indicating the deletion as a dominant-negative mutation. Conclusions: The above results support the pathogenic role of the 15 bp deletion on GJA8 in the Chinese family and demonstrate targeted genes sequencing as a resolution to molecular diagnosis of CCs.
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- 2016
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11. Rapid Detection and Identification of Infectious Pathogens Based on High-throughput Sequencing
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Pei-Xiang Ni, Xin Ding, Yin-Xin Zhang, Xue Yao, Rui-Xue Sun, Peng Wang, Yan-Ping Gong, Jia-Li Zhou, Dong-Fang Li, Hong-Long Wu, Xin Yi, Ling Yang, and Yun Long
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Epstein-Barr Virus ,Next-generation Sequencing ,Whole Genome Sequencing ,Medicine - Abstract
Background: The dilemma of pathogens identification in patients with unidentified clinical symptoms such as fever of unknown origin exists, which not only poses a challenge to both the diagnostic and therapeutic process by itself, but also to expert physicians. Methods: In this report, we have attempted to increase the awareness of unidentified pathogens by developing a method to investigate hitherto unidentified infectious pathogens based on unbiased high-throughput sequencing. Results: Our observations show that this method supplements current diagnostic technology that predominantly relies on information derived five cases from the intensive care unit. This methodological approach detects viruses and corrects the incidence of false positive detection rates of pathogens in a much shorter period. Through our method is followed by polymerase chain reaction validation, we could identify infection with Epstein-Barr virus, and in another case, we could identify infection with Streptococcus viridians based on the culture, which was false positive. Conclusions: This technology is a promising approach to revolutionize rapid diagnosis of infectious pathogens and to guide therapy that might result in the improvement of personalized medicine.
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- 2015
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12. Relationship of serum vitamin D levels with diabetic microvascular complications in patients with type 2 diabetes mellitus
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Zhao, Wei-Jing, primary, Xia, Xin-Yi, additional, and Yin, Jun, additional
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- 2021
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13. Effect of poly(DL-lactide-co-glycolide) on scar formation after glaucoma filtration surgery
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DU, Li-qun, YANG, Hong-ling, WU, Xin-yi, WANG, Shen-guo, and LI, Yun
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- 2013
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14. Effect of Phosphorylated-Extracellular Regulated Kinase 1/2 Inhibitor on Retina from Light-induced Photoreceptor Degeneration
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Ding, Xin-Yi, Gu, Rui-Ping, Tang, Wen-Yi, Shu, Qin-Meng, Xu, Ge-Zhi, and Zhang, Meng
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Inflammation ,Male ,Light ,Tumor Necrosis Factor-alpha ,Retinal Degeneration ,Blotting, Western ,Interleukin-1beta ,lcsh:R ,lcsh:Medicine ,Apoptosis ,Retina ,Rats ,Rats, Sprague-Dawley ,In Situ Nick-End Labeling ,Animals ,Original Article ,Mitogen-Activated Protein Kinases ,Phosphorylation - Abstract
Background: The demonstrated role of mitogen-activated protein kinase (MAPK) in both cell apoptosis and the inflammation pathway makes it an attractive target for photoreceptor protection. The aim of this study was to investigate the protective effects of MAPK antagonists against photoreceptor degeneration and retinal inflammation in a rat model of light-induced retinal degeneration. Methods: Sprague Dawley rats were treated with intravitreal injections of MAPK antagonists, inhibitors of p-P38, phosphorylated-extracellular regulated kinase (p-ERK) 1/2, and p-c-Jun N-terminal kinase (JNK) just before they were assigned to dark adaptation. After dark adaptation for 24 h, rats were exposed to blue light (2500 lux) in a light box for 24 h, and then returned to the normal 12-h light/12-h dark cycle. Samples were collected at different time points. MAPK expression during light exposure was examined with immunofluorescence. Photoreceptor death was detected with histopathology and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining. The expression of retinal p-ERK1/2, caspase 3, activated caspase 3, tumor necrosis factor (TNF)-α, and interleukin (IL)-1β was examined by Western blotting. Differences between groups were evaluated using unpaired one-way analysis of variance and least significant difference post hoc tests. Results: MAPKs (P38, ERK1/2, and p-JNK) were phosphorylated and activated in the light injury groups, compared with normal group, and their expressions were mainly elevated in the outer nuclear layer (ONL). Among the selected MAPK antagonists, only the p-ERK1/2 inhibitor attenuated the loss of photoreceptors and the thinning of ONL in light injury groups. Besides, p-ERK1/2 inhibitor refrained light-induced photoreceptor apoptosis, which was presented by TUNEL positive cells. Light injury significantly increased the expression of p-ERK1/2 (1.12 ± 0.06 vs. 0.57 ± 0.08, t = 9.99, P < 0.05; 1.23 ± 0.03 vs. 0.57 ± 0.08, t = 11.90, P < 0.05; and 1.12 ± 0.12 vs. 0.57 ± 0.08, t = 9.86, P < 0.05; F = 49.55, P < 0.001), and induced caspase 3 activating (0.63 ± 0.06 vs. 0.14 ± 0.05, t = 13.67, P < 0.05; 0.74 ± 0.05 vs. 0.14 ± 0.05, t = 16.87, P < 0.05; and 0.80 ± 0.05 vs. 0.14 ± 0.05, t = 18.57, P < 0.05; F = 100.15, P < 0.001), compared with normal group. The p-ERK1/2 inhibitor significantly reduced p-ERK1/2 overexpression (0.61 ± 0.06 vs. 1.12 ± 0.06, t = −9.26, P < 0.05; 0.77 ± 0.06 vs. 1.23 ± 0.03, t = −8.29, P < 0.05; and 0.68 ± 0.03 vs. 1.12 ± 0.12, t = −7.83, P < 0.05; F = 49.55, P < 0.001) and downregulated caspase 3 activating (0.23 ± 0.04 vs. 0.63 ± 0.06, t = −11.24, P < 0.05; 0.43 ± 0.03 vs. 0.74 ± 0.05, t = −8.86, P < 0.05; and 0.58 ± 0.03 vs. 0.80 ± 0.05, t = −6.17, P < 0.05; F = 100.15, P < 0.001), compared with light injury group. No significant change in the total level of caspase 3 was seen in different groups (F = 0.56, P = 0.75). As for inflammation, light injury significantly increased the expression of TNF-α (0.42 ± 0.04 vs. 0.25 ± 0.05, t = 5.99, P < 0.05; 0.65 ± 0.03 vs. 0.25 ± 0.05, t = 14.87, P < 0.05; and 0.86 ± 0.04 vs. 0.25 ± 0.05, t = 22.58, P < 0.05; F = 160.27, P < 0.001) and IL-1β (0.24 ± 0.01 vs. 0.19 ± 0.02, t = 2.33, P < 0.05; 0.35 ± 0.02 vs. 0.19 ± 0.02, t = 7.97, P < 0.05; and 0.48 ± 0.04 vs. 0.19 ± 0.02, t = 14.69, P < 0.05; F = 77.29, P < 0.001), compared with normal group. P-ERK1/2 inhibitor significantly decreased the overexpression of TNF-α (0.22 ± 0.02 vs. 0.42 ± 0.04, t = −7.40, P < 0.05; 0.27 ± 0.02 vs. 0.65 ± 0.03, t = −14.27, P < 0.05; and 0.33 ± 0.03 vs. 0.86 ± 0.04, t = −19.58, P < 0.05; F = 160.27, P < 0.001) and IL-1β (0.13 ± 0.03 vs. 0.24 ± 0.01, t = −5.77, P < 0.05; 0.17 ± 0.01 vs. 0.22 ± 0.02, t = −9.18, P < 0.05; and 0.76 ± 0.05 vs. 0.48 ± 0.04, t = −13.12, P < 0.05; F = 77.29, P < 0.001), compared with light injury group. Conclusion: The p-ERK1/2 inhibitor might protect the retina from light-induced photoreceptor degeneration and retinal inflammation.
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- 2018
15. A novel and feasible way to cultivate and purify endothelial progenitor cells from bone marrow of children with congenital heart diseases
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WU, Yong-tao, LI, Jing-xing, LIU, Shuo, XIN, Yi, WANG, Zi-jian, GAO, Jin, JI, Bing-yang, FAN, Xiang-ming, and ZHOU, Qi-wen
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- 2012
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16. In vivo biological stability of chemically pretreated silicone gel inserts intended for use in keratoprostheses
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DU, Li-qun, CHEN, Hong-mei, YAN, Ying, and WU, Xin-yi
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- 2012
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17. Treatment of depression using sleep electroencephalogram modulated repetitive transcranial magnetic stimulation
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HE, Ming-li, GU, Zheng-tian, WANG, Xin-yi, and SHI, Heng-ping
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- 2011
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18. Change of serum B-cell activating factor level in patients with positive antiphospholipid antibodies and previous adverse pregnancy outcomes and its significance
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Li, Xin-Yi, primary, Duan, Hong-Ji, additional, Liu, Xiang-Yuan, additional, and Deng, Xiao-Li, additional
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- 2020
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19. Chemokine-like factor-like MARVEL transmembrane domain-containing family in autoimmune diseases
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Duan, Hong-Ji, primary, Li, Xin-Yi, additional, Liu, Chang, additional, and Deng, Xiao-Li, additional
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- 2020
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20. Use of amniocytes for prenatal diagnosis of 22q11.2 microdeletion syndrome: a feasibility study
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LIU, Tao, LIU, Qing, WANG, Yi-xin, YANG, Dong, XIN, Yi, FANG, Zhen, DING, Shu-fang, and YANG, Jie-fu
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- 2010
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21. Proteolipid protein 1 gene mutation in nine patients with Pelizaeus-Merzbacher disease
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WANG, Jing-min, WU, Ye, WANG, Hui-fang, DENG, Yan-hua, YANG, Yan-ling, QIN, Jiong, LI, Xin-yi, WU, Xi-ru, and JIANG, Yu-wu
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- 2008
22. Triggering of toll-like receptors 2 and 4 by Aspergillus fumigatus conidia in immortalized human corneal epithelial cells to induce inflammatory cytokines
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ZHAO, Jie and WU, Xin-yi
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- 2008
23. Second pregnancy of trisomy 21 in a mother with mosaicism
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CUI, Ying-xia, HAO, Li-jun, WANG, Yun-hua, XIA, Xin-yi, SHI, Yi-chao, LU, Hong-yong, YAO, Bing, and HUANG, Yu-feng
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- 2007
24. Expression profiles and function of Toll-like receptors in human corneal epithelia
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WU, Xin-yi, GAO, Jian-lu, and REN, Mei-yu
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- 2007
25. The role of connective tissue growth factor, transforming growth factor β1 and Smad signaling pathway in cornea wound healing
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WU, Xin-yi, YANG, Yong-mei, GUO, Hui, and CHANG, Yuan
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- 2006
26. Skeletal Muscle Magnetic Resonance Imaging of the Lower Limbs in Late-onset Lipid Storage Myopathy with Electron Transfer Flavoprotein Dehydrogenase Gene Mutations
- Author
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Min-Ting Lin, Ning Wang, Dan-Ni Wang, Xin-Yi Liu, Jun-Jie He, Ming Jin, Hong-Xia Fu, and Zhi-Qiang Wang
- Subjects
Adult ,Male ,0301 basic medicine ,Adolescent ,Electron-Transferring Flavoproteins ,lcsh:Medicine ,Gene mutation ,Electron Transfer Flavoprotein Dehydrogenase ,Fat Infiltration Atrophy ,Late-onset Lipid Storage Myopathy ,Magnetic Resonance Imaging ,Multiple Acyl-coenzyme A Dehydrogenation Deficiency ,Medial compartment of thigh ,Lipid Metabolism, Inborn Errors ,Muscular Dystrophies ,Young Adult ,03 medical and health sciences ,0302 clinical medicine ,Atrophy ,medicine ,Humans ,Age of Onset ,Child ,Muscle, Skeletal ,Anterior compartment of thigh ,Muscle biopsy ,medicine.diagnostic_test ,business.industry ,lcsh:R ,Skeletal muscle ,Magnetic resonance imaging ,General Medicine ,Anatomy ,Middle Aged ,Posterior compartment of thigh ,musculoskeletal system ,medicine.disease ,body regions ,Muscular Atrophy ,030104 developmental biology ,medicine.anatomical_structure ,Lower Extremity ,Child, Preschool ,Mutation ,Original Article ,Female ,business ,030217 neurology & neurosurgery - Abstract
Background: Lipid storage myopathy (LSM) is a genetically heterogeneous group with variable clinical phenotypes. Late-onset multiple acyl-coenzyme A dehydrogenation deficiency (MADD) is a rather common form of LSM in China. Diagnosis and clinical management of it remain challenging, especially without robust muscle biopsy result and genetic detection. As the noninvasion and convenience, muscle magnetic resonance imaging (MRI) is a helpful assistant, diagnostic tool for neuromuscular disorders. However, the disease-specific MRI patterns of muscle involved and its diagnostic value in late-onset MADD have not been systematic analyzed. Methods: We assessed the MRI pattern and fat infiltration degree of the lower limb muscles in 28 late-onset MADD patients, combined with detailed clinical features and gene spectrum. Fat infiltration degree of the thigh muscle was scored while that of gluteus was described as obvious or not. Associated muscular atrophy was defined as obvious muscle bulk reduction. Results: The mean scores were significantly different among the anterior, medial, and posterior thigh muscle groups. The mean of fat infiltration scores on posterior thigh muscle group was significantly higher than either anterior or medial thigh muscle group (P < 0.001). Moreover, the mean score on medial thigh muscle group was significantly higher than that of anterior thigh muscle group (P < 0.01). About half of the patients displayed fat infiltration and atrophy in gluteus muscles. Of 28 patients, 12 exhibited atrophy in medial and/or posterior thigh muscle groups, especially in posterior thigh muscle group. Muscle edema pattern was not found in all the patients. Conclusions: Late-onset MADD patients show a typical muscular imaging pattern of fat infiltration and atrophy on anterior, posterior, and medial thigh muscle groups, with major involvement of posterior thigh muscle group and gluteus muscles and a sparing involvement of anterior thigh compartment. Our findings also suggest that muscle MRI of lower limbs is a helpful tool in guiding clinical evaluation on late-onset MADD.
- Published
- 2016
27. A Historical Cohort Study on the Efficacy of Glucocorticoids and Riboflavin Among Patients with Late-onset Multiple Acyl-CoA Dehydrogenase Deficiency
- Author
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Zhi-Qiang Wang, Xin-Yi Liu, Min-Ting Lin, Dan-Ni Wang, and Ning Wang
- Subjects
0301 basic medicine ,Adult ,Male ,medicine.medical_specialty ,Adolescent ,Riboflavin ,lcsh:Medicine ,Polymyositis ,Gastroenterology ,Lipid Metabolism, Inborn Errors ,Muscular Dystrophies ,Historical Cohort Study ,Cohort Studies ,03 medical and health sciences ,chemistry.chemical_compound ,Young Adult ,0302 clinical medicine ,Internal medicine ,medicine ,Late-onset Multiple Acyl-CoA Dehydrogenase Deficiency ,Humans ,Young adult ,Age of Onset ,Multiple Acyl-CoA Dehydrogenase Deficiency ,Child ,Multiple Acyl Coenzyme A Dehydrogenase Deficiency ,Glucocorticoids ,Lipid Storage Myopathy ,Muscle biopsy ,Fatty acid metabolism ,medicine.diagnostic_test ,business.industry ,lcsh:R ,digestive, oral, and skin physiology ,food and beverages ,General Medicine ,medicine.disease ,030104 developmental biology ,Endocrinology ,chemistry ,Original Article ,Female ,Age of onset ,business ,030217 neurology & neurosurgery ,Cohort study - Abstract
Background: Late-onset multiple acyl-CoA dehydrogenase deficiency (MADD) is the most common type of lipid storage myopathies in China. Most patients with late-onset MADD are well responsive to riboflavin. Up to now, these patients are often treated with glucocorticoids as the first-line drug because they are misdiagnosed as polymyositis without muscle biopsy or gene analysis. Although glucocorticoids seem to improve the fatty acid metabolism of late-onset MADD, the objective evaluation of their rationalization on this disorder and comparison with riboflavin treatment are unknown. Methods: We performed a historical cohort study on the efficacy of the two drugs among 45 patients with late-onset MADD, who were divided into glucocorticoids group and riboflavin group. Detailed clinical information of baseline and 1-month follow-up were collected. Results: After 1-month treatment, a dramatic improvement of muscle strength was found in riboflavin group (P < 0.05). There was no significant difference in muscle enzymes between the two groups. Significantly, the number of patients with full recovery in glucocorticoids group was less than the number in riboflavin group (P < 0.05). On the other hand, almost half of the patients in riboflavin group still presented high-level muscle enzymes and weak muscle strength after 1-month riboflavin treatment, meaning that 1-month treatment duration maybe insufficient and patients should keep on riboflavin supplement for a longer time. Conclusions: Our results provide credible evidences that the overall efficacy of riboflavin is superior to glucocorticoids, and a longer duration of riboflavin treatment is necessary for patients with late-onset MADD.
- Published
- 2016
28. Rhabdomyolysis and respiratory insufficiency due to the common ETFDH mutation of c.250G>A in two patients with late-onset multiple acyl-CoA dehydrogenase deficiency
- Author
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Chen, Hai-Zhu, primary, Jin, Ming, additional, Cai, Nai-Qing, additional, Lin, Xiao-Dan, additional, Liu, Xin-Yi, additional, Xu, Liu-Qing, additional, Lin, Min-Ting, additional, Lin, Feng, additional, Wang, Ning, additional, Wang, Zhi-Qiang, additional, and Xu, Guo-Rong, additional
- Published
- 2019
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29. Pharmacokinetic Studies of Factor VIII in Chinese Boys with Severe Hemophilia A
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Chen, Zhen-Ping, primary, Li, Pei-Jing, additional, Li, Gang, additional, Tang, Ling, additional, Zhen, Ying-Zi, additional, Wu, Xin-Yi, additional, Cheng, Xiao-Ling, additional, Luke, Koon Hung, additional, Blanchette, Victor S, additional, Poon, Man-Chiu, additional, Ding, Qiu-Lan, additional, and Wu, Run-Hui, additional
- Published
- 2018
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30. Proteolipid protein 1 gene mutation in nine patients with Pelizaeus-Merzbacher disease
- Author
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Ye Wu, Xin-Yi Li, Xiru Wu, Jiong Qin, Yuwu Jiang, Jingmin Wang, Yanling Yang, Yan-Hua Deng, and Huifang Wang
- Subjects
Genetics ,business.industry ,Point mutation ,Pelizaeus–Merzbacher disease ,General Medicine ,Gene mutation ,medicine.disease ,Molecular biology ,Gene dosage ,Exon ,Gene duplication ,Medicine ,Missense mutation ,Multiplex ligation-dependent probe amplification ,business - Abstract
BACKGROUND Pelizaeus-Merzbacher disease (PMD) is a rare X-linked recessive disorder with symptoms including nystagmus, impaired motor development, ataxia, and progressive spasticity. The proteolipid protein 1 (PLP1) gene is the only pathogenic gene of PMD. Duplication of the PLP1 gene is the most frequent gene defect, accounting for 50%-70% of PMD cases, whereas point mutations in the coding sequence or the splice sites account for 10%-25% of PMD cases. This study aimed to identify PLP1 mutations in nine unrelated Chinese patients (P1-9) with PMD, and 14 subjects from the family of patient 2 were also described. METHODS Genomic DNA was extracted from peripheral blood samples. Gene dosage was determined using the multiplex ligation-dependent probe amplification (MLPA). All 7 exons and exon-intron boundaries of the PLP1 gene were amplified and analyzed using direct DNA sequencing. RESULTS Of these nine patients, there were four transitional, four classical, and one connatal PMD according to their clinical and radiological presentations. PLP1 duplications were identified in patients 1-7 with PMD. Their mothers were PLP1 duplications carriers as well. Both duplication carriers and normal genotypes of PLP1 were identified in the family members of patient 2. A c.517C > T (p. P173S) hemizygous missense mutation in exon 4 was found in patient 8 with PMD, and his mother was shown to be a heterozygote of this mutation. CONCLUSIONS We identified seven genomic duplications and one missense mutation (p. P173S) of the PLP1 gene in eight Chinese patients with PMD. This is the report about PLP1 mutations in PMD patients from the mainland of China.
- Published
- 2008
31. Triggering of toll-like receptors 2 and 4 by Aspergillus fumigatus conidia in immortalized human corneal epithelial cells to induce inflammatory cytokines
- Author
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Xin-Yi Wu and Jie Zhao
- Subjects
Tumor Necrosis Factor-alpha ,Aspergillus fumigatus ,Interleukin-8 ,Epithelium, Corneal ,NF-kappa B ,General Medicine ,Biology ,biology.organism_classification ,Toll-Like Receptor 2 ,Proinflammatory cytokine ,Microbiology ,Toll-Like Receptor 4 ,TLR2 ,medicine.anatomical_structure ,Immune system ,medicine ,biology.protein ,Humans ,Secretion ,Antibody ,skin and connective tissue diseases ,Receptor ,Cells, Cultured ,Corneal epithelium - Abstract
BACKGROUND Cornea epithelial cells play early and crucial roles in the initiation of ocular surface responses to pathogens. Participation of toll-like receptor (TLR) 2 and TLR4, which are major forms of fungi receptors, may be involved in Aspergillus fumigatus induced immune responses. The objective of the present study was to examine whether inactive Aspergillus fumigatus conidia induce NF-kappaB activation and production of proinflammatory cytokines, and whether the expression of TLR2 and TLR4 were amplified by conidia in cultured immortalized human corneal epithelial cells (THCEs). This may contribute to our knowledge of the mechanism by which the host cornea can successfully defend against invasive fungi. METHODS Aspergillus fumigatus conidia were used to challenge THCE cells. THCE cells were harvested after 0.5, 1, 2 or 4 hours incubation. Real-time quantitative PCR was performed to determine the expression of TLR2, TLR4, TNF-alpha and IL-8. Western blotting was performed to determine the expression of NF-kappaB. Enzyme-linked immunosorbent assay (ELISA) was performed to determine the expression of TNF-alpha and IL-8. And the release of TNF-alpha and IL-8 in the cell supernatant were also assessed by ELISA with or without pretreatment with TLR2 and TLR4 neutralizing antibodies. RESULTS Aspergillus fumigatus conidia elicited the expression of TLR2, TLR4, TNF-alpha and IL-8 mRNA in THCEs. Exposure of THCE cells to Aspergillus fumigatus conidia resulted in NF-kappaB activation, which increased at 30 minutes (increased from 11.35+/-2.74 in the controls to 19.12+/-3.48, P
- Published
- 2008
32. Pediatric Infectious Endophthalmitis
- Author
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Zhang, Meng, primary, Xu, Ge-Zhi, additional, Jiang, Rui, additional, Ni, Ying-Qin, additional, Wang, Ke-Yan, additional, Gu, Rui-Ping, additional, and Ding, Xin-Yi, additional
- Published
- 2016
- Full Text
- View/download PDF
33. Rapid detection and identification of infectious pathogens based on high-throughput sequencing
- Author
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Xue Yao, Yinxin Zhang, Jiali Zhou, Peixiang Ni, Honglong Wu, Xin Yi, Ling Yang, Yanping Gong, Dongfang Li, Yun Long, Peng Wang, Ruixue Sun, and Xin Ding
- Subjects
Male ,Herpesvirus 4, Human ,Next-generation Sequencing ,lcsh:Medicine ,Epstein-Barr Virus ,Whole Genome Sequencing ,Biology ,Bioinformatics ,DNA sequencing ,law.invention ,law ,medicine ,Humans ,Fever of unknown origin ,Polymerase chain reaction ,Whole genome sequencing ,business.industry ,lcsh:R ,High-Throughput Nucleotide Sequencing ,General Medicine ,medicine.disease ,Viridans Streptococci ,Intensive care unit ,Human genetics ,Identification (biology) ,Female ,Original Article ,Personalized medicine ,business ,Epstein–Barr Virus - Abstract
Background: The dilemma of pathogens identification in patients with unidentified clinical symptoms such as fever of unknown origin exists, which not only poses a challenge to both the diagnostic and therapeutic process by itself, but also to expert physicians. Methods: In this report, we have attempted to increase the awareness of unidentified pathogens by developing a method to investigate hitherto unidentified infectious pathogens based on unbiased high-throughput sequencing. Results: Our observations show that this method supplements current diagnostic technology that predominantly relies on information derived five cases from the intensive care unit. This methodological approach detects viruses and corrects the incidence of false positive detection rates of pathogens in a much shorter period. Through our method is followed by polymerase chain reaction validation, we could identify infection with Epstein-Barr virus, and in another case, we could identify infection with Streptococcus viridians based on the culture, which was false positive. Conclusions: This technology is a promising approach to revolutionize rapid diagnosis of infectious pathogens and to guide therapy that might result in the improvement of personalized medicine.
- Published
- 2015
34. Effect of poly(DL-lactide-co-glycolide) on scar formation after glaucoma filtration surgery
- Author
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Li-qun, DU, Hong-ling, Yang, Xin-yi, Wu, Shen-guo, Wang, and Yun, Li
- Subjects
Cicatrix ,Polylactic Acid-Polyglycolic Acid Copolymer ,Filtering Surgery ,Animals ,Biocompatible Materials ,Glaucoma ,Lactic Acid ,Rabbits ,Polyglycolic Acid - Abstract
Glaucoma filtering surgery (GFS) is the most common procedure performed in the treatment of glaucoma. Although antiscarring agents help prevent postsurgical scarring and improve glaucoma surgical outcomes, they may be associated with an increased incidence of severe and potentially blinding complications. Poly(DL-lactide-co-glycolide) (PDLLA/GA) is a bioresorbable polymer, which can be prepared with a large range of physical, mechanical, and biological properties and has been widely used in medicine, including as an absorbable suture and a drug carrier and especially as a scaffold in tissue engineering. This study aimed to evaluate the effect of PDLLA/GA on scar formation after glaucoma filtration surgery (GFS).Forty-eight New Zealand white rabbits were divided into two groups randomly and GFS was performed on the right eye of each. PDLLA/GA membranes were put under the sclera flap for evaluation. GFS with no membrane inserted served as control. Clinical evaluations of intraocular pressure (IOP) and the presence of a filtration bleb were performed at intervals (3 days, 1, 2, 4, 8, 12, 20, and 24 weeks) postoperatively. At each time point, three eyes per group were excised to observe histological changes such as inflammation and scar formation and the expression of collagen type IV, proliferating cell nuclear antigen (PCNA), matrix metalloproteinase-9 (MMP-9), and tissue inhibitor of metalloproteinase-1 (TIMP-1). The expression of connective tissue growth factor (CTGF) mRNA was determined by reverse transcription-polymerase chain reaction.The lower IOP level and an effective bleb were maintained for a long time after GFS in the PDLLA/GA group. The histological analysis showed less inflammation and scar formation, weaker expression of collagen type IV and PCNA, more intense MMP-9 and TIMP-1, slightly elevated ratio of MMP-9 and TIMP-1, and a smaller increase in CTGF mRNA postoperatively in the PDLLA/GA group but less than the control group (P0.05).PDLLA/GA membranes may be promising for preventing fibrosis after GFS.
- Published
- 2013
35. Skeletal Muscle Magnetic Resonance Imaging of the Lower Limbs in Late-onset Lipid Storage Myopathy with Electron Transfer Flavoprotein Dehydrogenase Gene Mutations
- Author
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Liu, Xin-Yi, primary, Jin, Ming, additional, Wang, Zhi-Qiang, additional, Wang, Dan-Ni, additional, He, Jun-Jie, additional, Lin, Min-Ting, additional, Fu, Hong-Xia, additional, and Wang, Ning, additional
- Published
- 2016
- Full Text
- View/download PDF
36. A Historical Cohort Study on the Efficacy of Glucocorticoids and Riboflavin Among Patients with Late-onset Multiple Acyl-CoA Dehydrogenase Deficiency
- Author
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Liu, Xin-Yi, primary, Wang, Zhi-Qiang, additional, Wang, Dan-Ni, additional, Lin, Min-Ting, additional, and Wang, Ning, additional
- Published
- 2016
- Full Text
- View/download PDF
37. In vivo biological stability of chemically pretreated silicone gel inserts intended for use in keratoprostheses
- Author
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Li-Qun, DU, Hong-Mei, Chen, Ying, Yan, and Xin-Yi, Wu
- Subjects
Cornea ,Silicone Gels ,Corneal Stroma ,Corneal Edema ,Microscopy, Electron, Scanning ,Animals ,Biocompatible Materials ,Prostheses and Implants ,Rabbits ,Porosity - Abstract
Pretreatment with chemical agents could alter the surface chemistry of the silicone gel, which makes it suitable for epithelial migration onto its surface and thus enhances the cytobiocompatibility. This study aimed to evaluate the biological response of the corneal stroma to porous silicone gel pretreated with different chemical agents in vivo.The porous silicone gels were treated with a mixed acid solution containing 23.2% H2SO4 and 0.8% K2Cr2O7 for 10 or 15 minutes or with 30% H2O2 for 15 minutes. Discs (4 mm in diameter) were inserted into interlamellar stromal pockets of New Zealand white rabbits and followed up for a period of 3 months. Clinical evaluations such as corneal infiltration, edema and neovascularization were performed daily. At 3 months, the fibroplasias and collagen deposition were examined under light and scanning electron microscopy (SEM) and by immunohistochemical analysis.Pretreatment of the discs obviously decreased conjunctival congestion, discharge, cornea edema, and the extent of neovascularization. More fibroblasts migrated into the pretreated discs than into the control, and collagen was deposited, indicating that the biocompatibility of the corneal replacements was enhanced by the chemical pretreatments. From immunohistochemical analysis, Type I collagen deposition in the pretreated silicone discs was greater than in the control.Chemical treatment of silicone gel is effective in decreasing rabbit corneal inflammation, encouraging fibroblast in-growth, and enhancing tissue compatibility. Pretreated gels show good biological stability when used as a skirt material in Keratoprosthesis (Kpros).
- Published
- 2012
38. Treatment of depression using sleep electroencephalogram modulated repetitive transcranial magnetic stimulation
- Author
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Ming-Li, He, Zheng-Tian, Gu, Xin-Yi, Wang, and Heng-Ping, Shi
- Subjects
Adult ,Male ,Depressive Disorder ,Adolescent ,Emotions ,Humans ,Electroencephalography ,Female ,Middle Aged ,Child ,Sleep ,Transcranial Magnetic Stimulation ,Aged - Abstract
As a treatment of depression, the efficacy of conventional repetitive transcranial magnetic stimulation (rTMS) is limited, and symptoms recurrence is easy to occur after the treatment. This study aimed to examine the efficacy and safety of sleep electroencephalogram modulated repetitive rTMS (SEM-rTMS) in the treatment of depression.After 7 days without psychoactive medication, 164 patients with clinically defined depression were randomly divided into 3 groups: SEM-rTMS group (n = 57), conventional rTMS (C-rTMS, n = 55) group and sham-rTMS group (n = 52). Every patient was treated with the corresponding method for 30 minutes everyday for 10 days. Before and after scores on the 24-item Hamilton rating scale for depression (HAMD-24) and the clinical outcome on the 10th day of therapy for all subjects were analyzed.Twenty-two cases in the SEM-rTMS group obtained improved mood as compared to 6 in the C-rTMS group and 2 in the sham-rTMS group (χ(2) = 15.89, P = 0.0004). After completion of the rTMS phase of the protocol, a (51 ± 5)% reduction of HAMD-24 scores from the baseline in the SEM-rTMS group was found compared with a (34 ± 4)% in the C-rTMS group (q = 26.09, P = 0.001) and a (14 ± 3)% in sham-rTMS group (q = 57.53, P = 0.000). The 88% total effective rate in the SEM-rTMS group was significantly higher than 68% in the C-rTMS group and 20% in the sham-rTMS group (χ(2) = 12.01, P = 0.0025). No significant side effects were noted.SEM-rTMS is an effective and safe way for treating depression with repetitive transcranial magnetic stimulation (ChiCTR-TRC-00000438).
- Published
- 2011
39. Proteolipid protein 1 gene mutation in nine patients with Pelizaeus-Merzbacher disease
- Author
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Jing-Min, Wang, Ye, Wu, Hui-Fang, Wang, Yan-Hua, Deng, Yan-Ling, Yang, Jiong, Qin, Xin-Yi, Li, Xi-Ru, Wu, and Yu-Wu, Jiang
- Subjects
Male ,Pelizaeus-Merzbacher Disease ,Child, Preschool ,Gene Duplication ,Mutation ,Humans ,Infant ,Female ,Sequence Analysis, DNA ,Myelin Proteolipid Protein ,Nucleic Acid Amplification Techniques - Abstract
Pelizaeus-Merzbacher disease (PMD) is a rare X-linked recessive disorder with symptoms including nystagmus, impaired motor development, ataxia, and progressive spasticity. The proteolipid protein 1 (PLP1) gene is the only pathogenic gene of PMD. Duplication of the PLP1 gene is the most frequent gene defect, accounting for 50%-70% of PMD cases, whereas point mutations in the coding sequence or the splice sites account for 10%-25% of PMD cases. This study aimed to identify PLP1 mutations in nine unrelated Chinese patients (P1-9) with PMD, and 14 subjects from the family of patient 2 were also described.Genomic DNA was extracted from peripheral blood samples. Gene dosage was determined using the multiplex ligation-dependent probe amplification (MLPA). All 7 exons and exon-intron boundaries of the PLP1 gene were amplified and analyzed using direct DNA sequencing.Of these nine patients, there were four transitional, four classical, and one connatal PMD according to their clinical and radiological presentations. PLP1 duplications were identified in patients 1-7 with PMD. Their mothers were PLP1 duplications carriers as well. Both duplication carriers and normal genotypes of PLP1 were identified in the family members of patient 2. A c.517CT (p. P173S) hemizygous missense mutation in exon 4 was found in patient 8 with PMD, and his mother was shown to be a heterozygote of this mutation.We identified seven genomic duplications and one missense mutation (p. P173S) of the PLP1 gene in eight Chinese patients with PMD. This is the report about PLP1 mutations in PMD patients from the mainland of China.
- Published
- 2008
40. The role of connective tissue growth factor, transforming growth factor beta1 and Smad signaling pathway in cornea wound healing
- Author
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Xin-yi, Wu, Yong-mei, Yang, Hui, Guo, and Yuan, Chang
- Subjects
Wound Healing ,Connective Tissue Growth Factor ,Smad Proteins ,Immunohistochemistry ,Collagen Type I ,Fibronectins ,Immediate-Early Proteins ,Transforming Growth Factor beta1 ,Transforming Growth Factor beta ,Animals ,Intercellular Signaling Peptides and Proteins ,RNA, Messenger ,Rabbits ,Corneal Injuries ,Signal Transduction - Published
- 2006
41. The role of NF-kappaB activation in lipopolysaccharide induced keratitis in rats
- Author
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Xin-yi, Wu, Shao-ping, Han, Mei-yu, Ren, Yuan, Chang, and Fu-xin, Yu
- Subjects
Aqueous Humor ,Keratitis ,Lipopolysaccharides ,Tumor Necrosis Factor-alpha ,Epithelium, Corneal ,NF-kappa B ,Animals ,RNA, Messenger ,Rats, Wistar ,Immunohistochemistry ,Rats - Abstract
Nuclear factor-kappa B (NF-kappaB) is elevated in regulating transcription of many cytokines and inflammatory mediators. The purpose of this study was to investigate the activation and the significance NF-kappaB in lipopolysaccharide (LPS) induced keratitis.LPS induced keratitis model was based on Wistar rats. At 0.5, 1, 3, 6, 12, 24 or 72 hours after LPS treatment, the rat corneas were observed with a slit lamp microscope, then the rats were sacrificed and their corneas were excised for routine histological analysis. The expression of NF-kappaB was detected with immunohistochemical staining. The change of tumour necrosis factors-alpha (TNF-alpha) mRNA expression was identified by reverse transcriptase polymerase chain reaction (RT-PCR).Histological findings demonstrated that LPS treated corneas showed significant changes in corneal structure. Corneal edema, pronounced inflammatory cells infiltration and inordinate collagen fibres were observed. Immunohistochemical results showed that the expression of NF-kappaB and its activation obviously increased after LPS treatment compared with the normal group and control group. Positive cells could be observed at 0.5 hour and peak expression of NF-kappaB was observed between 3 hours and 12 hours after infection, but returned to or approached normal level by 72 hours. RT-PCR showed that the level of TNF-alpha mRNA began to increase 0.5 hour after LPS treatment, peaked at 6 hours and then subsided by 72 hours. NF-kappaB had a positive correlation with the expression of TNF-alpha mRNA (r = 0.964, P0.01), both NF-kappaB and TNF-alpha had a strong positive correlation with the degree of inflammatory response in LPS treated corneas (r = 0.929, P0.01; r = 0.587, P0.05, respectively).The activation of NF-kappaB was increased in LPS treated corneas and was elevated in LPS induced keratitis by promoting overexpression of TNF-alpha mRNA. NF-kappaB may play an important role in the pathogenesis of LPS-associated keratitis in rats.
- Published
- 2005
42. Effect of chemical treatment of silicon gel on tissue compatibility
- Author
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Xin-yi, Wu, Ying, Yan, and Li-qun, Du
- Subjects
Silicon ,Histocompatibility ,Cell Adhesion ,Epithelium, Corneal ,Animals ,Hydrogen Peroxide ,Rabbits ,Gels ,Cell Division - Abstract
Silicon gel is unfavourable for cell attachment and growth. This study was to study if pretreating the surface of silicon gel with chemical agents affects the proliferation of epithelial cells.Silicon gel was made and treated with either mixed acid solution (containing 232 g/dm(3) of H(2)SO(4) and 8 g/dm(3) of K(2)Cr(2)O(7)) or 300 cm(3)/dm(3) peroxide for 5, 10, and 15 minutes or 10, 15, and 20 minutes, respectively. The cultured corneal epithelial cells were seeded onto those silicon gels and kept for 13 days. Immunohistochemical investigations were then carried out for integrin (alpha 6 or beta 4) and actin.Growth of the epithelial cells in silicon gels treated with mixed acid solution for 10 minutes and 15 minutes was much significant than that in the untreated gels. After a 12-hour culture, a small number of corneal epithelial cells were proliferated on the surface of the silicon gels that had been treated with peroxide for 15 minutes. After a 3-day culture, those cells were further proliferated and fused together. The corneal epithelial cells did not grow well in the silicon gels treated with peroxide for 10 or 20 minutes. Immunostaining revealed the expression of actin and integrin alpha 6 or beta 4 on the silicon gels that were treated with mixed acid solution for 10 minutes or peroxide for 15 minutes.Silicon gels treated either with mixed acid solution for 10 or 15 minutes or with peroxide for 15 minutes improves cell proliferation.
- Published
- 2004
43. Second pregnancy of trisomy 21 in a mother with mosaicism
- Author
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Bing Yao, Yi-Chao Shi, Yu-Feng Huang, Li-jun Hao, Xin-Yi Xia, Hong-Yong Lu, Ying-Xia Cui, and Yun-Hua Wang
- Subjects
Adult ,Gynecology ,medicine.medical_specialty ,Down syndrome ,Mosaicism ,Obstetrics ,Offspring ,business.industry ,Germline mosaicism ,General Medicine ,medicine.disease ,Polymerase Chain Reaction ,Second pregnancy ,Recurrence risk ,Pregnancy ,Recurrence ,medicine ,Humans ,Female ,Down Syndrome ,Trisomy ,business ,Maternal Age - Abstract
In the case of a previous offspring with trisomy 21, recurrence risk for Down syndrome is about 1%.It may be due to chance,but the possibility of germline mosaicism for trisomy 21 in one of the parents has important implications for the recurrence.Here we report a young healthy mother,who has a second pregnancy of trisomy 21.
- Published
- 2007
44. Pediatric Infectious Endophthalmitis: A 271-case Retrospective Study at a Single Center in China.
- Author
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Zhang M, Xu GZ, Jiang R, Ni YQ, Wang KY, Gu RP, and Ding XY
- Subjects
- Adolescent, Anti-Bacterial Agents therapeutic use, Child, Child, Preschool, China, Endophthalmitis drug therapy, Endophthalmitis pathology, Eye Infections, Fungal drug therapy, Eye Infections, Fungal pathology, Eye Injuries, Penetrating microbiology, Female, Fusarium pathogenicity, Humans, Infant, Male, Retina microbiology, Retrospective Studies, Staphylococcus pathogenicity, Streptococcus pathogenicity, Vitrectomy, Endophthalmitis microbiology, Eye Infections, Fungal microbiology
- Abstract
Background: Pediatric infectious endophthalmitis is a serious sight-threatening disease for children. The purpose of this study was to investigate the etiology, microbiological spectrum, and visual outcomes of infectious endophthalmitis in children at a single institution in China., Methods: It is a retrospective study of the medical records of all patients under 14 years of age with histories of infectious endophthalmitis, treated at a single institution from January 1, 2009 to January 1, 2015. The clinical characteristics, etiology, microbiological spectrum, and management, as well as the visual outcomes, were analyzed. The Kappa test and Chi-square test were used in the statistical evaluation., Results: A total of 271 children were identified, with a mean age of 5.61 ± 2.93 years (range 5 months to 14 years). Ocular trauma (94.8%) and previous ocular surgery (3.0%) were the most common etiologies. Overall, 147 (54.2%) cases had positive cultures, and 176 organisms were isolated from these patients. A single species was isolated in 120 (81.6%) cases, with multiple organisms in 27 (18.4%) cases, and the most commonly identified organisms were coagulase-negative Staphylococcus and Streptococcus species, comprising 29.5% and 26.8% of the isolates, respectively. Moreover, of 176 isolates, 142 (80.8%) were Gram-positive organisms, 23 (13.0%) were Gram-negative organisms, and 11 (6.2%) were fungi. The final visual outcomes were 20/200 or better in 66 (24.4%) eyes, counting fingers to 20/200 in 34 (12.5%), hand motions in 30 (11.1%), light perception in 33 (12.2%), no light perception in 32 (11.8%), and 9 (3.3%) eyes were enucleated or eviscerated. The visual outcomes were not available in 67 (24.7%) patients., Conclusions: Penetrating ocular trauma is the most frequent cause of pediatric endophthalmitis in China. Streptococcus and Staphylococcus species are the most commonly identified organisms in exogenous pediatric endophthalmitis whereas Fusarium species are commonly seen in endogenous endophthalmitis. In this research, in spite of aggressive management with antibiotics and vitrectomy, the visual prognosis was found to be generally poor., Competing Interests: There are no conflicts of interest.
- Published
- 2016
- Full Text
- View/download PDF
45. The role of connective tissue growth factor, transforming growth factor beta1 and Smad signaling pathway in cornea wound healing.
- Author
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Wu XY, Yang YM, Guo H, and Chang Y
- Subjects
- Animals, Collagen Type I genetics, Connective Tissue Growth Factor, Fibronectins analysis, Immediate-Early Proteins analysis, Immediate-Early Proteins genetics, Immunohistochemistry, Intercellular Signaling Peptides and Proteins analysis, Intercellular Signaling Peptides and Proteins genetics, RNA, Messenger analysis, Rabbits, Transforming Growth Factor beta analysis, Transforming Growth Factor beta genetics, Transforming Growth Factor beta1, Corneal Injuries, Immediate-Early Proteins physiology, Intercellular Signaling Peptides and Proteins physiology, Signal Transduction physiology, Smad Proteins physiology, Transforming Growth Factor beta physiology, Wound Healing physiology
- Published
- 2006
46. The role of NF-kappaB activation in lipopolysaccharide induced keratitis in rats.
- Author
-
Wu XY, Han SP, Ren MY, Chang Y, and Yu FX
- Subjects
- Animals, Aqueous Humor cytology, Epithelium, Corneal physiology, Immunohistochemistry, Keratitis pathology, NF-kappa B analysis, RNA, Messenger analysis, Rats, Rats, Wistar, Tumor Necrosis Factor-alpha genetics, Tumor Necrosis Factor-alpha physiology, Keratitis chemically induced, Lipopolysaccharides toxicity, NF-kappa B physiology
- Abstract
Background: Nuclear factor-kappa B (NF-kappaB) is elevated in regulating transcription of many cytokines and inflammatory mediators. The purpose of this study was to investigate the activation and the significance NF-kappaB in lipopolysaccharide (LPS) induced keratitis., Methods: LPS induced keratitis model was based on Wistar rats. At 0.5, 1, 3, 6, 12, 24 or 72 hours after LPS treatment, the rat corneas were observed with a slit lamp microscope, then the rats were sacrificed and their corneas were excised for routine histological analysis. The expression of NF-kappaB was detected with immunohistochemical staining. The change of tumour necrosis factors-alpha (TNF-alpha) mRNA expression was identified by reverse transcriptase polymerase chain reaction (RT-PCR)., Results: Histological findings demonstrated that LPS treated corneas showed significant changes in corneal structure. Corneal edema, pronounced inflammatory cells infiltration and inordinate collagen fibres were observed. Immunohistochemical results showed that the expression of NF-kappaB and its activation obviously increased after LPS treatment compared with the normal group and control group. Positive cells could be observed at 0.5 hour and peak expression of NF-kappaB was observed between 3 hours and 12 hours after infection, but returned to or approached normal level by 72 hours. RT-PCR showed that the level of TNF-alpha mRNA began to increase 0.5 hour after LPS treatment, peaked at 6 hours and then subsided by 72 hours. NF-kappaB had a positive correlation with the expression of TNF-alpha mRNA (r = 0.964, P < 0.01), both NF-kappaB and TNF-alpha had a strong positive correlation with the degree of inflammatory response in LPS treated corneas (r = 0.929, P < 0.01; r = 0.587, P < 0.05, respectively)., Conclusions: The activation of NF-kappaB was increased in LPS treated corneas and was elevated in LPS induced keratitis by promoting overexpression of TNF-alpha mRNA. NF-kappaB may play an important role in the pathogenesis of LPS-associated keratitis in rats.
- Published
- 2005
47. Effect of chemical treatment of silicon gel on tissue compatibility.
- Author
-
Wu XY, Yan Y, and Du LQ
- Subjects
- Animals, Cell Adhesion drug effects, Cell Division drug effects, Epithelium, Corneal drug effects, Gels, Hydrogen Peroxide pharmacology, Rabbits, Histocompatibility drug effects, Silicon pharmacology
- Abstract
Background: Silicon gel is unfavourable for cell attachment and growth. This study was to study if pretreating the surface of silicon gel with chemical agents affects the proliferation of epithelial cells., Methods: Silicon gel was made and treated with either mixed acid solution (containing 232 g/dm(3) of H(2)SO(4) and 8 g/dm(3) of K(2)Cr(2)O(7)) or 300 cm(3)/dm(3) peroxide for 5, 10, and 15 minutes or 10, 15, and 20 minutes, respectively. The cultured corneal epithelial cells were seeded onto those silicon gels and kept for 13 days. Immunohistochemical investigations were then carried out for integrin (alpha 6 or beta 4) and actin., Results: Growth of the epithelial cells in silicon gels treated with mixed acid solution for 10 minutes and 15 minutes was much significant than that in the untreated gels. After a 12-hour culture, a small number of corneal epithelial cells were proliferated on the surface of the silicon gels that had been treated with peroxide for 15 minutes. After a 3-day culture, those cells were further proliferated and fused together. The corneal epithelial cells did not grow well in the silicon gels treated with peroxide for 10 or 20 minutes. Immunostaining revealed the expression of actin and integrin alpha 6 or beta 4 on the silicon gels that were treated with mixed acid solution for 10 minutes or peroxide for 15 minutes., Conclusion: Silicon gels treated either with mixed acid solution for 10 or 15 minutes or with peroxide for 15 minutes improves cell proliferation.
- Published
- 2004
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