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BACKGROUND: Cigarette smoke contains a large number of types of oxygen free radicals and cytotoxic components. Passive smoking will impair respiratory and cardiovascular system functions, and result in oxidative damage of skeletal muscle and decreased exercise ability. OBJECTIVE: To investigate the effect of phellinus igniarius crude polysaccharides on the exercise capacity and free radical metabolism of skeletal muscle in mice suffering passive smoking, so as to provide ideas for the prevention and treatment of peroxidative damage of skeletal muscle and depression of exercise capacity in rats suffering passive smoking. METHODS: Twenty-one male Kunming mice were randomly assigned to three groups: gavage with phellinus igniarius crude polysaccharides and suffering passive smoking (phellinus group), gavage with distilled water and suffering passive smoking (control group), and only gavage with distilled water (blank group). After 4 consecutive weeks, the mice were forced to take an exhausted swimming, and sacrificed subsequently. Exhausted swimming time was recorded. The bilateral gastrocnemius muscle tissues were obtained, in which the vitality of superoxide dismutase, catalase, glutathion reductase and Ca2+ -Mg2+ -ATP and Na+ -K+ -ATP activity, and the concentration of malonaldehyde were measured. RESULTS AND CONCLUSION: (1) The swimming lime of mice in the control group was shorter than that in the blank group (P < 0.01). The swimming time of mice in the phellinus group was longer than that in the control group (P < 0.01). (2) Compared with the blank group, the vitality of superoxide dismutase, catalase, glutathion reductase and Ga2+ -Mg2+ -ATP and Na+ -K+ -ATP activity in the control group were decreased (P < 0.01 ), and the concentration of malonaldehyde was increased (P < 0.01 ). (3) When compared with the control group, the vitality of those metabolic index in the phellinus group were increased (P < 0.05, P < 0.01 ), and the concentration of malonaldehyde was decreased (P < 0.01). (4) Correlation analysis showed that exhausted swimming time was positively correlated with the vitality of glutathion reductase, catalase and superoxide dis mutase (r > 0.6, P < 0.05), and was negatively correlated with the concentration of malonaldehyde (r=-0.637, P < 0.05). ca+2 -Mg+2 -ATP and Na+ -K+ -ATP activities were positively correlated with the vitality of glutalhion reductase, catalase and superoxide dismutase (r > 0.6, P < 0.05), and negatively correlated with the concentration of malonaldehyde (r < -0.6, P < 0.05). (5) In summary, phellinus igniarius crude polysaccharides can improve the antioxidative enzyme activity of skeletal muscle, inhibit lipid peroxidation reaction, and thus increase exercise ability of mice suffering passive smoking. The study was approved by the Ethical Committee of Jiangxi Normal University, approval No. 201703. [ABSTRACT FROM AUTHOR]