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National nature reserves are an important part of classification management on ecological protection in China. Taking the national nature reserves of Jilin Province as examples, this paper introduced the stability index by referring to the intensity model of land-use and land-cover change (LUCC), and analyzed the overall stability of land-use and land-cover (LULC) in the study area from the time interval level and land category level. The stability of LULC in different types of nature reserves was tracked and identified by extracting land-use change trajectory, and the land-use change trajectory was divided into three types: stable type, sub-stable type and unstable type. The impact of LUCC on regional ecosystem services was studied by using hotspot analysis and gravity center analysis. The results showed that: 1) The LULC in the study area was stable on the whole, and the proportion of stable land area reached 86.08%. The intensity of LUCC showed an increasing trend in recent 20 years, and the conversion of cultivated land and construction land was active in continuous time interval. 2) The stability of LULC in forest ecological reserves and wildlife reserves in the eastern part of Jilin Province was the highest, while that in inland wetland reserves and geological relic reserves in the central and western part of Jilin Province was lower. 3) The LUCC in national nature reserves not only changed the value of its own ecosystem service function, but also affected the ecosystem service function of the whole region. The combination of intensity analysis and land-use change trajectory was used to identify the characteristics of stability of LULC in nature reserves, which was conducive to deeply understand the process of LUCC in national nature reserves and provided reasonable suggestions for regional ecological protection. [ABSTRACT FROM AUTHOR]