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Morphology governs function. Yet, understanding and controlling the emergence of morphology at the molecular level remains challenging. The difficulty in studying the early stage of morphology formation is due to its stochastic nature both spatially and temporally occurring at the nanoscale. This nature has been particularly detrimental for the application of optical spectroscopy. To overcome this problem, we have been developing new in situ/in vivo optical spectroscopy tools, which are label-free and non-invasive. This account highlights several examples of how optical spectroscopy can become an important tool in studying the birth of morphology., (Copyright 2024 Natercia Barbosa, Oscar Urquidi, Johanna Brazard, Takuji B. M. Adachi. License: This work is licensed under a Creative Commons Attribution 4.0 International License.)