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Crop productivity and soil health are limited by organic carbon (OC), however, the variations in the mechanisms of SOC preservation in a complete soil profile subjected to long-term fertilization remains unclear. The objective of the study was to examined the content and profile distribution of the distinctive SOC protection mechanisms on a complete profile (0–100 cm) of Eumorthic Anthrosols in Northwest China after 23 years of chemical and manure fertilization. The soil was fractionated by combined physical-chemical and density floatation techniques. Throughout the profile, significant variations were observed among fractions. In the topsoil (0–20 and 20–40 cm), mineral coupling with the fertilization of manure (MNPK) enhanced total SOC content and recorded for 29% of SOC in the 0–20 and 20–40 cm layers. Moreover, MNPK increased the SOC content of the unprotected cPOC fraction by 60.9% and 61.5% in the 0–20 and 20–40 cm layer, while SOC content was low in the subsoil layers (40–60, 60–80 and 80–100 cm, respectively) compared with the control (C). The highest OC under MNPK in physically protected micro-aggregates (μagg) (6.36 and 6.06 g C kg−1), and occluded particulate organic carbon (iPOC) (1.41 and 1.29 g C kg−1) was found in the topsoil layers. The unprotected cPOC fraction was the greatest C accumulating fraction in the topsoil layers, followed by μagg and H-μSilt fractions in the soil profile, implying that these fractions were the most sensitive to the fertilization treatments. Overall, the unprotected, physically protected, and physico-chemically protected fractions were the dominant fractions for the sequestration of carbon across fertilization treatments and soil layers. Image 1 • Physical protection mechanisms were the most dominant in soil profile. • CPOC was the most sensitive fractions. • MNPK and SNPK caused accretion of total SOC content in a soil profile. • The correlations suggested that this soil has not yet reached C saturation. [ABSTRACT FROM AUTHOR]