Search

Immobilizing electro-active microbes within polymer matrices (thereby forming biohybrids) is a promising approach to accelerate microbial attachment to electrodes and increase the biofilm robustness. However, little is known on the fine scale chemical environment that develops within the electro-active biohybrids. Herein, we develop a biohybrid by immobilizing a culture of Shewanella oneidensis MR1 in agar matrix on the surface of a graphite electrode poised at +0.25 V. The resulting bioanode (3-6 mm thick) was grown under anoxic conditions and produced a steady current of 40 μA. Oxygen and pH distribution within the biohybrid were characterized in-situ using microsensors. As Shewanella is a facultative aerobe, it will halt the current production in the presence of oxygen. Thus, in addition, we investigated the alteration of the microenvironment during and after aeration of the medium to evaluate the oxygen tolerance of the system. During aeration, oxygen was effectively consumed in the top layers of the biofilm, leaving a 400-900 μm thick anoxic zone on the anode surface, that sustained >60% of the initial current. Current production recovered to pre-oxic condition within 5 h after the aeration was stopped, showing that immobilization can promote both high resistance and resilience of the system. Despite the absence of strong buffering conditions, pH profiles indicated a maximum drop of 0.2 units across the biohybrid. Characterizing the chemical microenvironment helps to elucidate the mechanistic functioning of artificial biofilms and hold a great potential for the designing of future, more effective biohybrid electrodes., Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests:Ramya Veerubhotla reports financial support was provided by Poul Due Jensen Foundation. Ugo Marzocchi reports financial support was provided by Poul Due Jensen Foundation. Ugo Marzocchi reports financial support was provided by Villum Foundation., (Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.)

Total Dissolved Sulfide (TDS) concentrations can either be derived from simultaneous measurement of pH and one of the sulfide species or determined indirectly in samples following an acidification step. Here we report a microsensor that allows for direct measurement of TDS in aquatic media without the need for pH monitoring. An acidic chamber placed in front of a commercial, amperometric H 2 S microsensor allows for the in-situ conversion of dissolved ionic sulfide species to H 2 S, which in turn is oxidized at the transducer anode. A typical sensor had a tip opening of 30 μm, a response time of <50 s and linear range between 0.5 and 650 μM. The sensor performance can be largely tuned by altering the geometry of the chamber. Sensors of different sensitivity (0.04-2.93 pA/μM) showed no noticeable change in zero current and sensitivity during continuous polarization over 7 weeks. The sensor was successfully applied to resolve microscale TDS gradients in freshwater and marine sediments. Other avenues of application include the online monitoring of industrial and urban sewers., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)