1. A relevant exposure to a food matrix contaminated environmentally by polychlorinated biphenyls induces liver and brain disruption in rats
- Author
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Anaïs Vénisseau, Christine Demeilliers, Florence Privé, Patricia Salen, Bruno Le Bizec, Isabelle Favier-Hininger, Eric Fontaine, Michel de Lorgeril, Cécile Batandier, Philippe Marchand, Frédéric Lamarche, Fayçal Ounnas, Laboratory of Fundamental and Applied Bioenergetics = Laboratoire de bioénergétique fondamentale et appliquée (LBFA), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Physiologie cardio-Respiratoire Expérimentale Théorique et Appliquée (TIMC-IMAG-PRETA), Techniques de l'Ingénierie Médicale et de la Complexité - Informatique, Mathématiques et Applications, Grenoble - UMR 5525 (TIMC-IMAG), Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Laboratoire d'étude des Résidus et Contaminants dans les Aliments (LABERCA), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS), Centre Hospitalier Universitaire [Grenoble] (CHU), Environnement et Prédiction de la Santé des Populations (TIMC-IMAG-EPSP), Laboratoire de bioénergétique fondamentale et appliquée (LBFA), Université Grenoble Alpes (UGA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Joseph Fourier - Grenoble 1 (UJF), and Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP)-IMAG-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP)-IMAG-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)
- Subjects
Male ,0301 basic medicine ,Tolerable daily intake ,[SDV]Life Sciences [q-bio] ,Health, Toxicology and Mutagenesis ,Respiratory chain ,010501 environmental sciences ,medicine.disease_cause ,01 natural sciences ,Rats, Sprague-Dawley ,Toxicology ,Lipid peroxidation ,chemistry.chemical_compound ,PCBs ,Respiratory system ,Chemistry ,Brain ,General Medicine ,Polychlorinated Biphenyls ,Pollution ,Mitochondria ,3. Good health ,Milk ,Liver ,Toxicity ,Food matrix ,Environmental Pollutants ,Female ,medicine.medical_specialty ,Environmental Engineering ,No-observed-adverse-effect level ,Food Contamination ,[SDV.TOX.TCA]Life Sciences [q-bio]/Toxicology/Toxicology and food chain ,Transaminase ,03 medical and health sciences ,Internal medicine ,medicine ,Animals ,Environmental Chemistry ,0105 earth and related environmental sciences ,No-Observed-Adverse-Effect Level ,Dose-Response Relationship, Drug ,Public Health, Environmental and Occupational Health ,General Chemistry ,Rats ,Oxidative Stress ,Low dose ,030104 developmental biology ,Endocrinology ,Lipid Peroxidation ,Oxidative stress - Abstract
International audience; Polychlorinated biphenyls (PCBs) are ubiquitous environmental contaminants present in dietary fats. Most studies evaluating PCB effects have been conducted with a single compound or a mixture of PCBs given as a single acute dose. The purpose of this study was to evaluate in vivo PCB toxicity in a realistic model of exposure: a low daily dose of PCBs (twice the tolerable daily intake (TDI)), chronically administered (8 weeks) to rats in contaminated goat milk. Liver and brain PCB toxicities were investigated by evaluating oxidative stress status and mitochondrial function. PCB toxicity in the liver was also estimated by transaminase enzymatic activity. This study shows that even at low doses, chronic PCB exposure resulted in a statistically significant reduction of mitochondrial function in liver and brain. In the liver, oxygen consumption in the condition of adenosine triphosphate (ATP) production (state 3) decreased by 22-29% (p < 0.01), according to the respiratory substrates. In the brain, respiratory chain complexes II and III were reduced by 24% and 39%, respectively (p < 0.005). The exposed rats presented higher lipid peroxidation status (+20%, p < 0.05) and transaminase activity (+30%, p < 0.05) in the blood. Thus, our study showed that exposure of rats to a daily realistic dose of PCBs (twice the TDI in a food complex mixture of environmental origin) resulted in multiple disruptions in the liver and brain.
- Published
- 2016