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Groundwater, a major source of drinking water worldwide, is often contaminated with micropollutants. Although microbial communities in aquifers and soils have the capability to biodegrade some micropollutants, this process is limited in situ. Biostimulation with dissolved organic carbon (DOC) is known to promote micropollutant biodegradation, but the role of DOC biodegradability is still poorly understood. This study investigated how three DOC types with different biodegradability (humics, dextran and acetate) affect the biodegradation of 15 micropollutants by aquifer and soil microbial communities under aerobic and nitrate reducing conditions. Although originating from different environments, both communities were able to biodegrade the same 4 micropollutants under aerobic conditions - 2,4-D, MCPP, chloridazon (CLZ) and chloridazon-desphenyl. However, DOC addition only affected MCPP biodegradation, promoting MCPP biodegradation regardless of DOC biodegradability. Biodegradation of 2,4-D, MCPP and CLZ under aerobic conditions was observed after a lag phase, whose duration differed per compound. 2,4-D was biodegraded first and fully. Aquifer community was able to degrade about half of the initial MCPP concentration (removal efficiency of 49.3 ± 11.7%). CLZ was fully biodegraded by the aquifer community, but not by the soil community, possibly due to substrate competition with organics originating from the inoculum. Therefore, the natural organic carbon present in the inocula and in environmental systems can influence micropollutant biodegradation. Under nitrate reducing conditions micropollutant biodegradation was not observed nor biostimulated by DOC addition. The results also highlight the importance of sufficient exposure time to trigger in situ micropollutant biodegradation., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 The Authors. Published by Elsevier Ltd.. All rights reserved.)