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The fraction transformation from stable to mobile forms in sediments is continuous, slow, and spontaneous chain reactions causing static risks to the aquatic system. However, this process may change into abrupt, rapid, and dynamic paths when certain physicochemical conditions changed. Using the Delayed Geochemical Hazard (DGH) model, comprehensive methods combing both static and dynamic risk assessment were therefore conducted to evaluate the aforementioned processes. By applying these methods, arsenic (As) pollution in surface sediments of the Baiyangdian Lake (BYD Lake) was investigated thoroughly as a case study area. The results showed that the total As concentrations in those sediment samples ranged from 4.87 to 17.94 mg/kg, with an average of 8.75 mg/kg. In a fraction, Fe and Mn were observed to pose effects on the surface-adsorbed (As S) and residual fractions (As R) with the coefficient analysis. The static risk assessment showed that both the contamination and ecological risk are at a low level in the total content but a low to moderate risk in the fraction. The dynamic risk assessment posted the potential transformation paths of As in the sediments, indicating a trend of potential DGH burst in 45.24%–78.57% of the BYD Lake. In summary, this study provides a methodology for the risk assessment of arsenic that may extend to other heavy metal(loid)s combining static and dynamic processes in sediments. • As content and fraction in surface sediments were investigated in Baiyangdian Lake. • Comprehensive risks including static and dynamic risk were assessed synchronously. • Static risk of As was analyzed by employing four indices (I geo , ICF, E r i , and RAC). • DGH model provided potential transformation paths of As for revealing the dynamic risk. [ABSTRACT FROM AUTHOR]

Beijing parks always have a large flow of local residents and tourists, and the soil Pb could threaten human health by incidental ingestion. Soil samples from eleven parks in Beijing were collected to assess the human health risk associated with Pb. Lead bioaccessibility in these parks ranged from 3.2 ± 0.4% to 12.1 ± 0.5% in the physiologically based extraction test (PBET) gastric phase and increased when approaching the city center. The chemical forms and soil properties (Fe, organic matter, and grain size) were important factors affecting the soil Pb bioaccessibility. The geo-accumulation index of Beihai Park (BH, near the city center) reached 1.3 ± 0.1 indicating moderate contamination. Lead health risk to children in BH should be of concern though its hazard quotient was below one. Results obtained from the Diffusive Gradients in Thin-films (DGT)-induced fluxes in the soils (DIFS) model showed that Pb-release in some parks farther from the city center was a "partially sustained case" (R diff < R < 0.95) indicating that soil particles could partially replenish effective Pb to the soil solution. A relatively higher desorption rate constant (k 1) and shorter characteristic response time (T c) were also found in these parks, indicating non-negligible release risk. Soil Pb based on the PBET method and DIFS model could provide a reliable reference to park managers for the health risk management of Pb pollution. Image 1 • Lead pollution and risk in urban park soils were systematically investigated. • In vitro methods were used to assess Pb accumulation and health risk. • DIFS model was used to study Pb resupply capacity in urban park soils. [ABSTRACT FROM AUTHOR]

The human health risk associated with arsenic in food in Southeast Brazil was quantified. Based on the most commonly consumed food types in the Brazilian diet, the maximum inorganic As (iAs) daily intake from food (0.255 μg kg −1 body weight per day) is approximately 9% of the Benchmark Dose Lower Limit (BMDL 0.5 ) of 3 μg kg −1 body weight per day set by the World Health Organization (WHO) and Food and Agriculture Organization (FAO) Joint Expert Committee in Food Additives (JECFA). When water is included, the contribution of food to the total intake varies from 96.9% to 39.7%. Rice and beans, the main Brazilian staple food, contribute between 67 and 90% of the total As intake from food (46–79% from rice and 11–23% from beans). The substantial contribution of beans to total As food intake is reported for the first time. The broad range of As concentrations in rice and beans highlights the variable and potentially large contribution of both to As food intake in places where diet consists largely of these two food categories. [ABSTRACT FROM AUTHOR]

Mixed contamination of benzo[ a ]pyrene (B[ a ]P), arsenic (As), cadmium (Cd), and lead (Pb) is a major environmental and human health concern. The mixture toxicity data on these co-contaminants are important for their risk assessment. In this study, we have determined the mixture toxicity of As, Cd and Pb, and B[ a ]P with As, Cd or Pb in HepG2 cells. The binary mixtures of Cd + As, Cd + Pb and As + Pb and B[ a ]P + metals (B[ a ]P + As, B[ a ]P + Cd and B[ a ]P + Pb) were evaluated for their interaction on the cytotoxicity using the MTS assay. A full factorial design (4 × 5) was used to determine the interaction toxicity and all the six mixtures showed significant interaction on the cytotoxicity. We further investigated the role of oxidative stress (reactive oxygen species (ROS) generation) and antioxidant defense mechanism (total glutathione (GSH) level) with the observed cytotoxicity. The mixtures of metals reduced the total GSH level and increased the ROS generation, respectively. In the case of mixtures of B[ a ]P and metals, both total GSH level and ROS generation were increased. Overall, the binary mixtures of metals and B[ a ]P with metals caused a dose dependent toxicity to HepG2 cells. The results also showed a significant contribution of oxidative stress to the observed toxicity and the potential protective role of the total GSH level against this mixture toxicity. The findings of interaction between B[ a ]P and metals might have an impact on the potential human health risk of this mixtures at contaminated sites. [ABSTRACT FROM AUTHOR]

Cytotoxicity of benzene, toluene, ethylbenzene and xylenes (BTEX) to human lung cells was explored using three different exposure methods: Method 1 – in normal 96-well plates using DMSO as a carrier vehicle, we exposed (a) human lung carcinoma A549 cells, (b) A549 cells over-expressed with cytochrome P450 2E1 cells, and (c) normal lung fibroblast LL-24 cells to benzene, toluene, ethylbenzene and xylene individually and in a mixture which models car exhaust gases for between 1–88 h. We found that the order of the BTEX potency is benzene < toluene < ethylbenzene = m- xylene with acute BTEX toxicity to A549 ≈ LL-24 > CYP2E1 over-expressed A549 cells. A significant difference was found between inter-assay responses for all 24 h exposures ( P < 0.005) suggesting a poor assay repeatability. No sign of potency increase was found from 6 to 72 h exposures. Method 2 – Using sealed vials to expose A549 cells to benzene, toluene and ethylbenzene, we observed a twenty-fold increase in their cytotoxicity, but also with no time-course effect. Method 3 – Using air exposed hanging-drop cell culture, we were able to see both an increase of demonstration of toxicity and a time-course effect from 1 to 12 h exposure. We conclude that exposing cells in sealed and unsealed media using DMSO as a carrier vehicle was not suitable for BTEX exposure studies. Hanging-drop air exposure has more potential. It should be noted that if there are any changes in their exposure matrixes, its exposure mass distribution in cells could differ. [ABSTRACT FROM AUTHOR]

Mine wastes contain a mixture of metals and metalloids including arsenic (As) and cadmium (Cd). This study investigated the potential interaction between As and Cd in a rat model. Sprague Dawley rats were dosed with sodium arsenate via the oral (0, 0.5, 5 and 15mg As kg−1 b.w.) or intravenous (0.5mg As kg−1 b.w.) route to establish its dose-response relationship in terms of bioavailability and pharmacokinetic parameters. Bioavailability of As reduced when the dose of As increased. For the interaction study a fixed oral dose of As at 2.5mg As kg−1 b.w. solo and in combination with Cd as cadmium chloride at 3 or 6mg Cd kg−1 b.w. were administered to rats. Bioavailability of As was decreased by 34-35% in the presence of Cd. Elimination half-life of As was also decreased from 69 days in the As solo group to 13-22days in the presence of 3 and 6mg Cd kg−1 b.w. respectively. Decreased urinary excretion of As and tissue accumulation were also observed. A probable explanation for these findings is that As co-administration with Cd could have resulted in the formation of less soluble cadmium-arsenic complexes in the guts of the rats. Nevertheless, such an interaction between As and Cd could only explained about 44-48% of the variation when mine waste materials containing both of these elements were administered to rats. This suggests other physical properties and chemical compound formation could contribute to the observed bioavailability of arsenic in complex environmental samples. [ABSTRACT FROM AUTHOR]

Atrazine (AZ) is an environmental endocrine disrupting chemical which can affect the development of amphibians. In our past studies, we demonstrated that chronical exposure to 100 μg/L AZ can cause abnormalities in development and related genes expression of gonads in developing male Xenopus laevis ( X. laevis ) tadpoles. Recent studies by others have demonstrated that circular RNAs (circRNAs) are implicated in multiple developmental anomalies. However, whether circRNAs involve in the effects in AZ-exposed X. laevis remains unknown. In this study, over 68575 circRNAs were detected by circRNA sequencing of testis tissues from control groups ( n  = 3) and AZ-treated X. laevis ( n  = 3). Treatment of AZ led to 405 circRNAs differentially expressed including 44 upregulated and 361 downregulated compared with froglets in the control groups. Two upregulated and 6 downregulated circRNAs were further validated by real-time PCR assay which displayed consistent regulation patterns as shown by the transcriptome sequencing results. Two hundreds and eighty two differentially expressed circRNAs played miRNA sponges roles. Kyoto Encyclopedia of Genes and Genomes pathway analysis of miRNA targets showed that AZ-affected circRNAs are mainly involved in 19 pathways. The Wnt signaling pathway and progesterone-mediated oocyte maturation pathway may be two involved signal pathways. This study for the first time provides evidence that AZ can alter circRNAs which play a role in AZ-induced testicular degeneration of developing male X. laevis through regulation of expressions of functional genes in the testes of X. laevis. [ABSTRACT FROM AUTHOR]

As a widely used herbicide, atrazine (AZ) has been extensively studied for its adverse effects on the reproductive system, especially feminization in male animals. However, the relationship of gene expression changes and associated toxicological endpoints remains unclear. In this study, developing Xenopus laevis tadpoles were exposed to concentration of AZ at 0.1, 1, 10 or 100 μg/L continuously. Compared with froglets in the control group, there were no significant differences in body length, body weight, liver weight and hepatosomatic index (HSI) of males in groups treated with AZ for 90 d. At 100 μg/L AZ treatment caused a significant reduction of gonad weight and gonadosomatic index (GSI) of males ( p < 0.01). In addition, AZ at all dose levels caused testicular degeneration, especially in froglets from the groups with 0.1 and 100 μg/L which exhibited U-shaped dose-response trend. We further investigated the gene expression changes associated with the testicular degeneration induced by AZ. We found that the expression of 1165 genes was significantly altered with 616 upregulated and 549 downregulated compared to the expression profile of the control animals. KEGG analysis showed that genes which were significantly affected by AZ are mainly involved in arginine and proline metabolism, cell cycle, riboflavin metabolism, spliceosome, base excision repair and progesterone-mediated oocyte maturation pathway. Our results show that AZ may affect reproductive and immune systems by interference with the related gene expression changes during the male X. laevis development. The findings may help to clarify the feminization mechanisms of AZ in male X. laevis . [ABSTRACT FROM AUTHOR]

The bioaccessibility of lead (Pb) in contaminated soils has been extensively studied, including the influence of soil properties on Pb bioaccessibility. However, little is known about the effects of other metals/metalloid, such as arsenic (As), cadmium (Cd) on the bioaccessibility of Pb, i.e. whether As or Cd could increase or decrease the solubility of Pb in human gastrointestinal tract when Pb-contaminated soil and As-contaminated (or Cd-contaminated) soil are ingested simultaneously. Furthermore, it is far from clear that if soil property could make a difference to these effects. In this study, seven types of soils were collected in Australia and spiked with As, Cd or Pb. Gastric bioaccessibility of Pb ranged from 44 ± 0.9% to 100 ± 6.7% whilst intestinal bioaccessibility dropped to 1 ± 0.2% to 36 ± 1.7%. Statistical analysis shows total Pb in soil was the most significant controller for bioaccessible Pb. Effects of As and Cd on the bioaccessibility of Pb in simulated human digestive system were studied by mixing As-spiked soil (or Cd-spiked soil) with Pb-spiked soil of the same type during bioaccessibility test. Results reveal that neither As nor Cd had impact on Pb bioaccessibility, which indicates when As, Cd and Pb aged in soils separately, they may behave independently in the bioaccessibility measuring system. This finding can be part of evidence to assume additive effect when it comes to estimate the bioaccessibility of mixtures of independently-aged As and Pb (or Cd and Pb) in soils. [ABSTRACT FROM AUTHOR]

Recent decades have seen a growing popularity of in vitro bioaccessibility being utilised as a screening tool in human health risk assessment. However the existing bioaccessibility studies only focus on single contaminant. Considering human are likely to ingest multi-contaminants, these contaminants could interact within human gastrointestinal tract which may lead to an increase or decrease in bioaccessibility. In this study, seven different types of soil were spiked with arsenic (As) or cadmium (Cd) and aged for one year. The effects of soil properties on the bioaccessibility were examined. Moreover, the interaction between As and Cd in simulated human digestive system was studied by mixing As-spiked soil with Cd-spiked soil of the same type during bioaccessibility test. Results shows the bioaccessibility of As ranged from 40 ± 2.8 to 95 ± 1.3% in the gastric phase and 16 ± 2.0 to 96 ± 0.8% in the intestinal phase whilst a significant difference was observed between Cd gastric bioaccessibility (72 ± 4.3 to 99 ± 0.8%) and intestinal bioaccessibility (6.2 ± 0.3 to 45 ± 2.7%). Organic carbon, iron oxide and aluminium oxide were key parameters influencing the bioaccessibility of As (gastric and intestinal phases) and Cd (intestinal phase). No interactions between As and Cd during bioaccessibility test were observed in any soils, which indicates As and Cd may age independently and did not interact while being solubilised during bioaccessibility test. Thus additive effect may be proposed when estimating the bioaccessibility of mixtures of independently-aged As and Cd in soils. [ABSTRACT FROM AUTHOR]