1. Iron Coordination Properties of Gramibactin as Model for the New Class of Diazeniumdiolate Based Siderophores.
- Author
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Gama S, Hermenau R, Frontauria M, Milea D, Sammartano S, Hertweck C, and Plass W
- Abstract
Gramibactin (GBT) is an archetype for the new class of diazeniumdiolate siderophores, produced by Paraburkholderia graminis, a cereal-associated rhizosphere bacterium, for which a detailed solution thermodynamic study exploring the iron coordination properties is reported. The acid-base behavior of gramibactin as well as its complexing ability toward Fe
3+ was studied over a wide range of pH values (2≤pH≤11). For the latter the ligand-competition method employing EDTA was used. Only two species are formed: [Fe(GBT)]- (pH 2 to 9) and [Fe(GBT)(OH)2 ]3- (pH≥9). The formation of [Fe(GBT)]- and its occurrence in real systems was confirmed by LC-HRESIMS analysis of the bacteria culture broth extract. The sequestering ability of gramibactin was also evaluated in terms of the parameters pFe and pL0.5 . Gramibactin exhibits a higher sequestering ability toward Fe3+ than EDTA and of the same order of magnitude as hydroxamate-type microbial siderophores, but smaller than most of the catecholate-type siderophores and much higher than the most known phytosiderophores., (© 2020 The Authors. Chemistry - A European Journal published by Wiley-VCH GmbH.) more...- Published
- 2021
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