1. Light-Activated Carbon Monoxide Prodrugs Based on Bipyridyl Dicarbonyl Ruthenium(II) Complexes.
- Author
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Geri S, Krunclova T, Janouskova O, Panek J, Hruby M, Hernández-Valdés D, Probst B, Alberto RA, Mamat C, Kubeil M, and Stephan H
- Subjects
- Cell Line, Tumor, HEK293 Cells, Humans, 2,2'-Dipyridyl chemistry, Carbon Monoxide chemistry, Prodrugs chemistry, Prodrugs radiation effects, Ruthenium chemistry
- Abstract
Two photoactivatable dicarbonyl ruthenium(II) complexes based on an amide-functionalised bipyridine scaffold (4-position) equipped with an alkyne functionality or a green-fluorescent BODIPY (boron-dipyrromethene) dye have been prepared and used to investigate their light-induced decarbonylation. UV/Vis, FTIR and
13 C NMR spectroscopies as well as gas chromatography and multivariate curve resolution alternating least-squares analysis (MCR-ALS) were used to elucidate the mechanism of the decarbonylation process. Release of the first CO molecule occurs very quickly, while release of the second CO molecule proceeds more slowly. In vitro studies using two cell lines A431 (human squamous carcinoma) and HEK293 (human embryonic kidney cells) have been carried out in order to characterise the anti-proliferative and anti-apoptotic activities. The BODIPY-labelled compound allows for monitoring the cellular uptake, showing fast internalisation kinetics and accumulation at the endoplasmic reticulum and mitochondria., (© 2020 The Authors. Published by Wiley-VCH GmbH.)- Published
- 2020
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