Search

The fraction of hydrophobic peptides was eluted with MeCN (70%) in trifluoroacetic acid (TFA) solution (0.05%). The antibacterial activity of the culture fluid (Table 1) and intermediate fractions in each purification step was determined by spotting on agar and estimating the diameter of the indicator culture growth inhibition zone [[6]]. Extension of tryptic fragment sequences of bacteriocin Mal from Lactobacillus plantarum strain Mal and known orthologs from similar strains. [Extracted from the article]

The accumulation dynamics of cellulolytic enzymes in culture media of the basidiomycete fungi Panus tigrinus, Pleurotus ostreatus, Fomes fomentarus, and the micromycete Aspergillus terreus were studied during a long incubation period. It was found that A. terreus was the most active producer of cellulolytic enzymes among the studied fungi. Two protein fractions with cellulase activity were isolated using gel filtration and ion-exchange chromatography. PAAG electrophoresis showed that fraction-I consisted of four components; fraction-II, an electrophoretically homogeneous protein. [ABSTRACT FROM AUTHOR]

The naturally occurring octacyclopeptide brachystemin F was synthesized for the first time in the present study by a two-step solid-phase/solution strategy. All the amino acids in this study have the L-configuration. The linear octapeptide was assembled by standard Fmoc solid-phase peptide synthesis (SPPS), and subsequent cyclization was carried out by the solution method. The target compound was purified by preparative RP-HPLC, and its structure was identified by H NMR, C NMR, and MS. [ABSTRACT FROM AUTHOR]

The article presents a phytochemical investigation on the chemical constituents of Alisma orientalis, a Alismataceae genus that is widely cultivated in China and Japan. The compounds include altaicalarin B, alisol A and arnidiol. The plant materials used in the investigation and the detailed extract, isolation, and purification process are outlined.

The article provides information on the study on the ixeridium gracile plant. The plant is a herbaceous perennial plant which belongs to the Compositae family and is used as a representative Tibetan herbal medicine. The study involves the isolation and purification of eleven triterpenoids compounds and three steroids from an alcoholic extract of the whole plant of ixeridium gracile.

The article focuses on the separation and purification of daphnetin, daphnodorin B, p-hydroxybenzoic acid, and daphnodorin D 1 in Daphne odora var. Margirmt plant through high-speed counter-current chromatography (HSCCC). It explains that Daphne odora var. Margirmt is a flower and medical plant from the family of Thymelaeaceae. It cites how the dried plant was chopped into small pieces and extracted with a solvent mixture that composed of 95% ethyl acetate and ethanol.