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By the use of oligonucleotides bearing a terminal monophosphate, 10-base gap was formed in substrate DNA and the monophosphates were placed at the edge of gap. Upon the treatment of these systems w...

DNA hydrolysis by the Ce(IV)/EDTA complex is enormously accelerated by the cooperation with selected oligoamines. The reaction in the presence of ethylenediamine (100 mmol dm−3) is 50 times as fast as that in its absence.

Homogeneous Ce(IV) complex of EDTA promptly hydrolyzes oligonucleotides under physiological conditions. In contrast, dinucleotides are not hydrolyzed to measurable extents. The degree of polymerization of DNA substrate is crucial for the present catalysis.