1. Characterization of PEGylated Asparaginase: New Opportunities from NMR Analysis of Large PEGylated Therapeutics
- Author
-
Enrico Ravera, Giacomo Parigi, Azzurra Carlon, Claudio Luchinat, Stefano Giuntini, Marco Fragai, Linda Cerofolini, and Vito Calderone
- Subjects
Magnetic Resonance Spectroscopy ,Combinatorial chemistry ,Hydrolase ,Computational biology ,PEGylation ,biopharmaceuticals ,protein modifications ,protein structures ,structural biology ,010402 general chemistry ,01 natural sciences ,Catalysis ,Polyethylene Glycols ,Protein structure ,Coated Materials, Biocompatible ,Asparaginase ,010405 organic chemistry ,Chemistry ,Organic Chemistry ,Rational design ,General Chemistry ,Integrated approach ,0104 chemical sciences ,3. Good health ,Characterization (materials science) ,Structural biology ,Pegylated asparaginase ,Protein Multimerization ,Peg coating - Abstract
Resonance assignment and structural characterization of pharmacologically relevant proteins promise to improve understanding and safety of these proteins by rational design. However, the PEG coating that is used to evade the immune system also causes these molecules to "evade" the standard structural biology methodologies. We here demonstrate that it is possible to obtain the resonance assignment and a reliable structural model of large PEGylated proteins through an integrated approach encompassing NMR and X-ray crystallography.
- Published
- 2019
- Full Text
- View/download PDF