1. Carbohydrate-Derived Metal-Chelator-Triggered Lipids for Liposomal Drug Delivery.
- Author
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Holmstrøm, Thomas, Malle, Mette Galsgaard, Shunliang Wu, Jensen, Knud Jørgen, Hatzakis, Nikos S., and Pedersen, Christian Marcus
- Subjects
LIPIDS ,LIPOSOMES ,ETHYLENEDIAMINETETRAACETIC acid ,FLUORESCENCE microscopy ,LIPID synthesis ,FREIGHT & freightage - Abstract
Liposomes are versatile three-dimensional, biomaterial- based frameworks that can spatially enclose a variety of organic and inorganic biomaterials for advanced targeted-delivery applications. Implementation of external-stimulicontrolled release of their cargo will significantly augment their wide application for liposomal drug delivery. This paper presents the synthesis of a carbohydrate-derived lipid, capable of changing its conformation depending on the presence of Zn
2+ : an active state in the presence of Zn2+ ions and back to an inactive state in the absence of Zn2+ or when exposed to Na2 EDTA, a metal chelator with high affinity for Zn2+ ions. This is the first report of a lipid triggered by the presence of a metal chelator. Total internal reflection fluorescence microscopy and a single-liposome study showed that it indeed was possible for the lipid to be incorporated into the bilayer of stable liposomes that remained leakage-free for the fluorescent cargo of the liposomes. On addition of EDTA to the liposomes, their fluorescent cargo could be released as a result of the membrane-incorporated lipids undergoing a conformational change. [ABSTRACT FROM AUTHOR]- Published
- 2021
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