1. A Chemical Biology Approach to Interrogate Quorum-Sensing Regulated Behaviors at the Molecular and Cellular Level
- Author
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Colin A. Lowery, Susana Matamouros, Jonathan Scolnick, Gunnar F. Kaufmann, Jie Zhu, Jenny M. Lively, Kim D. Janda, Sherry Niessen, Benjamin F. Cravatt, and Samuel I. Miller
- Subjects
Proteomics ,Salmonella typhimurium ,Cell signaling ,Clinical Biochemistry ,Chemical biology ,Molecular Probe Techniques ,Biology ,Biochemistry ,Article ,Microbiology ,Lactones ,Bacterial Proteins ,Pentanones ,Gene expression ,Drug Discovery ,Homoserine ,RNA, Messenger ,Molecular Biology ,Pharmacology ,Quorum Sensing ,General Medicine ,Small molecule ,Cell biology ,Quorum sensing ,Proteome ,Molecular Medicine ,Intracellular - Abstract
SummarySmall molecule probes have been used extensively to explore biologic systems and elucidate cellular signaling pathways. In this study, we use an inhibitor of bacterial communication to monitor changes in the proteome of Salmonella enterica serovar Typhimurium with the aim of discovering unrecognized processes regulated by AI-2-based quorum-sensing (QS), a mechanism of bacterial intercellular communication that allows for the coordination of gene expression in a cell density-dependent manner. In S. typhimurium, this system regulates the uptake and catabolism of intercellular signals and has been implicated in pathogenesis, including the invasion of host epithelial cells. We demonstrate that our QS antagonist is capable of selectively inhibiting the expression of known QS-regulated proteins in S. typhimurium, thus attesting that QS inhibitors may be used to confirm proposed and elucidate previously unidentified QS pathways without relying on genetic manipulation. more...
- Published
- 2013
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