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6-Chloro-9-(β-D-arabinofuranosyl)purine served as an intermediate for the chemical synthesis of a series of 6-substituted selenopurine arabinosides. In an in vitro test using murine leukemic cells (L-5178Y) these 6-substituted selenopurine arabinosides showed some cytotoxicity. Lengthening the side chain had no effect on their cytotoxicity. Selenourea was a useful reagent for synthesizing selenopurines, selenopurine nucleosides, and selenopurine arabinoside under mild conditions.

Treatment of adenosine, 2-aminoadenosine, adenine arabinoside, formycin, 1-Me-AMP, cyclo-AMP, and 1-Me-cyclo-AMP with H2Se in aqueous pyridine gave the corresponding seleno compounds with good yield.

8-Bromoguanosine cyclic 3',5'-monophosphate, 8-bromoguanosine 5'-monophosphate, and 8-bromoguanosine served as intermediates for the chemical synthesis of a series of 8-substituted seleno cyclic nucleotides, nucleotides, and their nucleosides. Selenourea was found to be a useful reagent in synthesizing these seleno-substituted nucleoside, nucleotide, and cyclic nucleotide. A nucleic acid analyzer was used to study the hydrolysis of these cyclic nucleotides by phosphodiesterase. It was found that all of the 8-substituted selenoguanosine cyclic 3',5'-phosphates synthesized, except 8-MeSe-cGMP, were resistant to hydrolyze by phosphodiesterase. These 8-substituted seleno cyclic GMP derivatives showed some antitumor activities against murine leukemic cells (L5178Y) in vitro and in vivo.