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A home-made silica-based silver ion solid-phase extraction (Ag+-SPE) system for the fractionation and subsequent gas chromatographic analysis of trans fatty acids in human adipose tissue is developed and examined. Analytical characteristics of the home-made Ag+-SPE column were compared with those of the commercial Discovery Ag-Ion SPE column and it was demonstrated that the both columns can be applied for the fractionation of fatty acid methyl esters. [ABSTRACT FROM AUTHOR]

Silver ion solid-phase extraction is suggested for the analysis of trans fatty acids in human adipose. Acid catalyzed esterification of the fatty acids protocol using boron trifluoride-methanol complex as a catalyst was used. Silica based Ag+-SPE conditions are presented. A mixture of dichloromethane and hexane with the content of dichloromethane increasing from 5 to 100% was used as an eluent. The presented method allows eliminating from the trans chromatographic zone about 75% of cis isomers of fatty acid methyl esters, thus enabling to determine trace contents of trans fatty acids in the samples containing big amounts of dominant saturated, monounsaturated and polyunsaturated fatty acids. [ABSTRACT FROM AUTHOR]