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In this work the combination of several analytical techniques, such as Fourier transform infrared spectroscopy (FTIR), nuclear magnetic resonance (NMR) spectroscopy, scanning electron microscopy (SEM) and metal content determination using atomic absorption spectroscopy (AAS), was used for the characterization of adipose tissue samples taken from volunteer obese patients. The obtained results provided information about the adipose tissue chemical and structural composition of adipose tissue layers in the human body, as well as the main microstructural features. It was demonstrated for the first time to the best of our knowledge that these methods are indispensable tools in order to investigate some special features of the human adipose tissue, identifying its chemical composition and structure. From the obtained results we concluded that such characterization of the adipose tissue is an essential step for the possible prediction of appearance of symptoms of different diseases. [ABSTRACT FROM AUTHOR]