1. DVL, lectin from Dioclea violacea seeds, has multiples mechanisms of action against Candida spp via carbohydrate recognition domain.
- Author
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Silva RRS, Malveira EA, Aguiar TKB, Neto NAS, Roma RR, Santos MHC, Santos ALE, Silva AFB, Freitas CDT, Rocha BAM, Souza PFN, and Teixeira CS
- Subjects
- Candida metabolism, Plant Lectins pharmacology, Plant Lectins metabolism, Antifungal Agents pharmacology, Carbohydrates, Seeds metabolism, Ergosterol, Candida albicans, Microbial Sensitivity Tests, Lectins pharmacology, Dioclea metabolism
- Abstract
Lectins are proteins of non-immunological origin with the ability to bind to carbohydrates reversibly. They emerge as an alternative to conventional antifungals, given the ability to interact with carbohydrates in the fungal cell wall inhibiting fungal growth. The lectin from D. violacea (DVL) already has its activity described as anti-candida in some species. Here, we observed the anti-candida effect of DVL on C. albicans, C. krusei and C. parapsilosis and its multiple mechanisms of action toward the yeasts. Additionally, it was observed that DVL induces membrane and cell wall damage and ROS overproduction. DVL was also able to cause an imbalance in the redox system of the cells, interact with ergosterol, inhibit ergosterol biosynthesis, and induce cytochrome c release from the mitochondrial membrane. These results endorse the potential application of DVL in developing a new antifungal drug to fight back against fungal resistance., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)
- Published
- 2023
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