1. Biotransformation of phorbol by human intestinal bacteria
- Author
-
Norio Nakamura, Masao Hattori, and Atef A. Abdel-Hafez
- Subjects
Magnetic Resonance Spectroscopy ,biology ,Bacteria ,Chemistry ,Croton tiglium ,Euphorbiaceae ,General Chemistry ,General Medicine ,Metabolism ,Pharmacognosy ,biology.organism_classification ,Phorbols ,Intestines ,chemistry.chemical_compound ,Biochemistry ,Pharmacokinetics ,Biotransformation ,Drug Discovery ,Phorbol ,Humans - Abstract
Anaerobic incubation of phorbol (1) from Croton tiglium with human intestinal bacteria afforded five metabolites: isophorbol (2), deoxyphorbol (3), 4beta,9alpha,20-trihydroxy-13,15-seco-1,6,15-tigliatriene-3,13-dione (4), 4beta,9alpha,20-trihydroxy-15,16,17-trinor-1,6-tigliadiene-3,13-dione (5) and 4beta,9a,20-trihydroxy-14(13-->12)-abeo-12alphaH-1,6-tigliadiene-3,13-dione (6). All these metabolites (2-6) were identified and characterized by spectroscopic means, including two-dimensional (2D)-NMR. Nine defined strains from the human intestine showed an ability to transform 1 to these metabolites.
- Published
- 2002