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Abstract: To understand the production mechanisms for multiply charged ions C q + (q =2–3) and I q + (q =2–4), the kinetic energy of released electron was measured for understanding the interaction of nanosecond laser with CH3I cluster. The mean electron energy increased from 19 to 33eV as the laser intensity was raised from 2.9×109 to 4.7×1010 W/cm2. The evolution of the mean electron energy as a function of laser intensity was similar to that of multiply charged ions, which indicated that electron impact ionization played an important role in the formation of C q + (q =2–3) and I q + (q =2–4). Moreover, the formation of more ionized sites would lead to the cluster splitting, which is disadvantageous for the multiply charged ion production. [Copyright &y& Elsevier]