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Developing sensing platforms that simultaneously integrate high sensitivity and accuracy has been a promising but challenging task for the detection of protein biomarkers in clinical samples. Herein, we engineered an Au nanostar-based liquid phase interfacial ratiometric SERS platform with programmable entropy-driven DNA circuits to detect the protein biomarker Mucin 1 (MUC1) in clinical samples.

Developing convenient and sensitive detection methods for antibiotic residues in food is beneficial for ensuring food quality and human health. The tough challenges that limit the development of sensitive, quantitative, portable, on-the-spot antibiotic detectors are the lack of simple and effective target recognition and signal amplification strategies, and direct digital quantification. Herein, we developed a visual digital quantitative aptasensor, based on a binding-induced 3-D DNA nanomachine signal probe, for the simple and sensitive, on-the-spot detection of antibiotics.

The tough challenges for the sensitive and accurate detection of circulating miRNAs (cmiRNAs) in the peripheral blood are low abundance, and high interference from environmental or background factors or other molecules. Here, we developed a double-signal mode based on metal-organic framework coupled cascaded nucleic acid circuits for the accurate and sensitive detection of serum circulating miRNAs.

The tough challenges for the convenient and quantitative determination of circulating miRNAs (cmiRNAs) in the peripheral blood are low abundance, high interference and lack of direct digital readout. Here, we developed dual-enhanced magnetobiosensors based on cascaded nucleic acid circuits, which integrate catalyzed hairpin assembly (CHA) with the hybridization chain reaction (HCR), for sensitive, portable and digital quantitative detection of circulating miRNAs in serum by a personal glucose meter (PGM).

Development of reliable and affordable quantitative methods for miRNAs with high specificity and sensitivity is a central challenge to make miRNA testing a routine part of medical care with respect to cancer. Herein, we propose a strategy for glucoamylase-encapsulated liposome-encoded magnetic beads initiated by padlock exponential rolling circle amplification (P-ERCA) for portable and accurate quantification of miRNA by using a glucometer readout.

Here we developed a simple, sensitive and accurate PLD detection method based on a target-controlled gating liposome (TCGL) "off-on" cascade amplified strategy and personal glucose meters (PGMs). It showed excellent sensitivity with a detection limit of 0.005 U L(-1) and well performed PLD activity analysis in breast cancer cells and inhibitor drug screening.

We developed a one-pot label-free molecular beacon-mediated quadratic isothermal exponential amplification strategy (LFMB-QIEA) for simple, rapid and sensitive DNA methyltransferase (MTase) activity detection.

A novel label-free fluorescent sensing scheme for sensitive and selective detection of NAD(+) and ATP has been developed based on dumbbell probe-mediated rolling circle amplification (D-RCA)-responsive G-quadruplex formation. This approach can detect 0.5 pM for ATP and 100 fM for NAD(+), much lower than those of previously reported biosensors, and exhibits high discrimination ability.

A single DNA molecule detection method on DNA tetrahedron decorated substrates has been developed. DNA tetrahedra were introduced onto substrates for both preventing nonspecific adsorption and sensitive recognition of single DNA molecules.

A novel rolling circle amplification (RCA) immunoassay based on DNA enriching magnetic nanoparticles and assembled fluorescent DNA nanotags, magnetic nanoparticles-RCA immunoassay, is developed as a versatile fluorescence assay platform for highly sensitive proteins detection.