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Start Over Author "D. Lucas Kerr" Journal chemical communications (cambridge, england)
1 results on '"D. Lucas Kerr"'

1. A self-immolative linker that releases thiols detects penicillin amidase and nitroreductase with high sensitivity

Author

Eli M, Espinoza, Joachim J, Røise, Maomao, He, I-Che, Li, Alvin K, Agatep, Patrick, Udenyi, Hesong, Han, Nicole, Jackson, D Lucas, Kerr, Dake, Chen, Michael R, Stentzel, Emily, Ruan, Lee, Riley, and Niren, Murthy

Subjects
Spectrometry, Fluorescence, Molecular Structure, Penicillin Amidase, Sulfhydryl Compounds, Nitroreductases, Article
Abstract

This article reports the synthesis and characterization of a novel self-immolative linker, based on thiocarbonates, which releases a free thiol upon activation via enzymes. We demonstrate that thiocarbonate self-immolative linkers can be used to detect the enzymes penicillin G amidase (PGA) and nitroreductase (NTR) with high sensitivity using absorption spectroscopy. Paired with modern thiol amplification technology, the detection of PGA and NTR were achieved at concentrations of 160 nM and 52 nM respectively. In addition, the PGA probe was shown to be compatible with both biological thiols and enzymes present in cell lysates.

Published
2023

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