1. Simple, rapid detection of influenza A (H1N1) viruses using a highly sensitive peptide-based molecular beacon
- Author
-
Kyeonghye Guk, Hyeran Kim, Eun Kyung Lim, Juyeon Jung, and Bong Hyun Chung
- Subjects
viruses ,education ,Hemagglutinin (influenza) ,Peptide ,Hemagglutinin Glycoproteins, Influenza Virus ,02 engineering and technology ,010402 general chemistry ,medicine.disease_cause ,01 natural sciences ,Rapid detection ,Sensitivity and Specificity ,Catalysis ,Influenza A Virus, H1N1 Subtype ,Molecular beacon ,Influenza, Human ,Materials Chemistry ,Influenza A virus ,medicine ,Fluorescence Resonance Energy Transfer ,Humans ,Detection limit ,chemistry.chemical_classification ,biology ,Metals and Alloys ,General Chemistry ,021001 nanoscience & nanotechnology ,Molecular biology ,Fluorescence ,0104 chemical sciences ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,Förster resonance energy transfer ,chemistry ,cardiovascular system ,Ceramics and Composites ,biology.protein ,0210 nano-technology ,Peptides ,circulatory and respiratory physiology - Abstract
A peptide-based molecular beacon (PEP-MB) was prepared for the simple, rapid, and specific detection of H1N1 viruses using a fluorescence resonance energy transfer (FRET) system. The PEP-MB exhibited minimal fluorescence in its "closed" hairpin structure. However, in the presence of H1N1 viruses, the specific recognition of the hemagglutinin (HA) protein of H1 strains by the PEP-MB causes the beacon to assume an "open" structure that emits strong fluorescence. The PEP-MB could detect H1N1 viruses within 15 min or even 5 min and can exhibit strong fluorescence even at low viral concentrations, with a detection limit of 4 copies.
- Published
- 2015