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Start Over Author "Hexin Xie" Journal chemical communications
4 results on '"Hexin Xie"'

1. Specific detection of IMP-1 β-lactamase activity using a trans cephalosporin-based fluorogenic probe

Author

Zheng Ma, Fangfang Chen, Hexin Xie, Chang Yuan, Tao Yu, Zhiyao Li, Yingqiu Zou, Liqiang Hu, and Runqiu Liu

Subjects
medicine.drug_class, Specific detection, Cephalosporin, Catalysis, Serine, polycyclic compounds, Materials Chemistry, medicine, Moiety, chemistry.chemical_classification, biology, Chemistry, Metals and Alloys, General Chemistry, biochemical phenomena, metabolism, and nutrition, bacterial infections and mycoses, Combinatorial chemistry, Enzyme assay, Surfaces, Coatings and Films, Electronic, Optical and Magnetic Materials, enzymes and coenzymes (carbohydrates), Enzyme, Reagent, Ceramics and Composites, biology.protein, bacteria, Selectivity
Abstract

A fluorogenic probe for the specific detection of IMP-1 β-lactamase activity has been developed. This imaging reagent features a unique trans-acetylamino cephalosporin as an enzymatic recognition moiety, exhibiting excellent selectivity to IMP-1 β-lactamase over other β-lactamases, including serine- and metallo-β-lactamases. The selective activation of the probe by IMP-1 β-lactamase leads to over 30-fold enhancement in the fluorescence intensity, which allows enzyme activity to be reported with high sensitivity.

Published
2021
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2. Late-stage difluoromethylation leading to a self-immobilizing fluorogenic probe for the visualization of enzyme activities in live cells

Author

Hexin Xie, Jialing Jiang, Qingwei Tan, Shuxuan Zhao, Liqiang Hu, and Heng Song

Subjects
010402 general chemistry, Methylation, 01 natural sciences, Catalysis, Materials Chemistry, Humans, Indolequinones, Fluorescent Dyes, chemistry.chemical_classification, 010405 organic chemistry, HEK 293 cells, Metals and Alloys, Late stage, General Chemistry, beta-Galactosidase, Fluorescence, 0104 chemical sciences, Surfaces, Coatings and Films, Electronic, Optical and Magnetic Materials, Fluorescence intensity, HEK293 Cells, Spectrometry, Fluorescence, Enzyme, Biochemistry, chemistry, Covalent bond, Reagent, Ceramics and Composites, HeLa Cells
Abstract

Reported herein is a novel p-quinone methide-based self-immobilizing fluorogenic probe for the visualization of β-galactosidase activities in live cells. This easily prepared imaging reagent massively increases the fluorescence intensity and covalently links to the activation site with high efficiency upon enzymatic trigger.

Published
2019
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3. Bioluminescent nanosensors for protease detection based upon gold nanoparticle–luciferase conjugatesElectronic supplementary information (ESI) available: Experimental section, the determination of the number of Luc8 per Au NP, signal recovery of Luc8 after the click reaction, the quenching efficiency of the conjugate, and time-dependent monitoring of MMP-2 activity. See DOI: 10.1039/b915612g

Author

Young-Pil Kim, Weston L. Daniel, Zuyong Xia, Hexin Xie, Chad A. Mirkin, and Jianghong Rao

Subjects
NANOSTRUCTURED materials, BIOSENSORS, BIOLUMINESCENCE, PROTEOLYTIC enzymes, COLLOIDAL gold, LUCIFERASES, BIOCONJUGATES
Abstract

This communication reports the use of click chemistry to site-specifically conjugate bioluminescent Renillaluciferase proteins to gold nanoparticles (Au NPs) for sensing protease activity. The bioluminescent emission from luciferase was efficiently quenched by Au NPs, but significantly recovered after the proteolytic cleavage. [ABSTRACT FROM AUTHOR]

Published
2010
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4. Highly enantio- and diastereoselective organocatalytic cascade aza-Michael–Michael reactions: a direct method for the synthesis of trisubstituted chiral pyrrolidinesElectronic supplementary information (ESI) available: Experimental and NMR data. CCDC reference number 693305. For ESI and crystallographic data in CIF or other electronic format see DOI: 10.1039/b812464g

Author

Hao Li, Liansuo Zu, Hexin Xie, Jian Wang, and Wei Wang

Subjects
ALDOL condensation, ORGANIC compounds, ENANTIOSELECTIVE catalysis, STEREOCHEMISTRY, CHEMICAL reactions, PYRROLIDINE
Abstract

An unprecedented highly enantio- and diastereoselective cascade aza-Michael–Michael reaction of α,β-unsaturated aldehydes with trans-γ-Ts protected amino α,β-unsaturated ester has been developed; the simple and practical process, efficiently catalyzed by chiral diphenylprolinol TMS ether, serves as a powerful access to highly functionalized trisubstituted chiral pyrrolidines. [ABSTRACT FROM AUTHOR]

Published
2008
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