1. Novel bioactive peptides demonstrating anti-dengue virus activity isolated from the Asian medicinal plant Acacia Catechu
- Author
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Prapaphan Budchart, Sittiruk Roytrakul, Pa-thai Yenchitsomanus, Atchara Paemanee, Kiattawee Choowongkomon, Petlada Yongpitakwattana, Siriluk Rattanabunyong, Sucheewin Krobthong, Nunghathai Sawasdee, and Aussara Panya
- Subjects
viruses ,Peptide ,Dengue virus ,Serogroup ,medicine.disease_cause ,Antiviral Agents ,01 natural sciences ,Biochemistry ,Virus ,Chlorocebus aethiops ,Drug Discovery ,medicine ,Animals ,Amino Acid Sequence ,Medicinal plants ,Acacia catechu ,Vero Cells ,Pharmacology ,chemistry.chemical_classification ,Plants, Medicinal ,Traditional medicine ,biology ,010405 organic chemistry ,Chemistry ,Organic Chemistry ,Acacia ,Dengue Virus ,biology.organism_classification ,0104 chemical sciences ,010404 medicinal & biomolecular chemistry ,Enzyme ,Food supplement ,Cell toxicity ,Molecular Medicine ,Peptides - Abstract
The therapeutic activities of food-derived bioactive proteins and peptides are attracting increased attention within the research community. Medicinal plants used in traditional medicines are an excellent source of bioactive proteins and peptides, especially those traditionally prepared by water extraction for use as tea or food supplement. In this study, novel bioactive peptides were isolated from enzymatic digests of 33 Thai medicinal plants. The inhibitory activity of each against dengue virus (DENV) infection was investigated. Of 33 plants, peptides from Acacia catechu extract demonstrated the most pronounced anti-DENV activity. Half maximal inhibitory concentration of 0.18 μg/ml effectively inhibited DENV foci formation. Treatment with 1.25 μg/ml crude peptide extract could reduce virus production less than 100-fold with no observable cell toxicity. Peptide sequences were determined by high-performance liquid chromatography and liquid chromatography-tandem mass spectrometry. Two bioactive peptides isolated from Acacia catechu inhibited DENV foci formation >90% at the concentration of 50 μM; therefore, they are recommended for further investigation as antiviral peptides against DENV infection.
- Published
- 2018
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