1. Peptide-grafted nanodiamonds: preparation, cytotoxicity and uptake in cells.
- Author
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Vial S, Mansuy C, Sagan S, Irinopoulou T, Burlina F, Boudou JP, Chassaing G, and Lavielle S
- Subjects
- Animals, CHO Cells, Cell Death drug effects, Cells cytology, Cricetinae, Cricetulus, Intracellular Space drug effects, Intracellular Space enzymology, Oxidoreductases metabolism, Cells drug effects, Cells metabolism, Diamond metabolism, Diamond toxicity, Nanoparticles chemistry, Oligopeptides chemistry
- Abstract
Nanodiamonds that were prepared by high pressure/high temperature were functionalized with biomolecules for biological applications. Nanodiamonds (NDs, < or =35 nm) that were coated by silanization or with polyelectrolyte layers were grafted with a fluorescent thiolated peptide via a maleimido function; this led to an aqueous colloidal suspension that was stable for months. These substituted NDs were not cytotoxic for CHO cells. Their capacity to enter mammalian cells, and their localisation inside were ascertained after labelling the nucleus and actin, by examining the cells by confocal, reflected light and fluorescence microscopy.
- Published
- 2008
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