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Introduction: Bloodstain pattern analysis (BPA) is a crucial tool in forensic investigations, particularly in cases that involve violent crimes such as homicides, assaults, and sexual assaults. The analysis of bloodstains can provide valuable information about the location and movement of the victim and perpetrator(s), the type of weapon used, and the number and direction of the blows or shots. The lack of an objective mathematical foundation is a critical issue in a scenario where the quality of evidences can strongly affect a court trial and the life of people involved in that trial. Systematic reviews provide a rigorous and transparent method for evaluating scientific evidence, and can help to identify strengths and weaknesses of a given methodology.Methods: To ensure comprehensive retrieval of relevant research we will search the following key databases: PubMed and ScienceDirect through 1000 for peer reviewed articles (in all languages) evidence related to bloodstain pattern analysis.Discussions: This systematic review summarizes four papers that discuss different aspects of BPA. The first paper introduced the basic concepts of BPA and the use of trigonometric principles to determine the impact angle of bloodstains. The second paper presented experimental findings on the use of trigonometry in BPA and identified its limitations in determining the height of blood sprayed upwards. The third paper examined the analysis of circular bloodstains and found that their clusters can provide indirect information on impact-to-wall distance and area of origin. The fourth paper introduced the use of virtual models for BPA and found that straight-line trajectories can be used to estimate the area of origin with high accuracy.Conclusions: These papers highlight the complexity of BPA and the importance of using a combination of methods to interpret bloodstain patterns accurately. It is essential to consider the limitations of each method and to use them in combination to obtain a more complete picture of the events that occurred at a crime scene. Further research is needed to refine and improve the methods used in BPA and to develop new approaches to analyzing bloodstain patterns. [ABSTRACT FROM AUTHOR]