1. Measles Virus Persistent Infection of Human Induced Pluripotent Stem Cells
- Author
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Solly Mizrahi, Rivka Ofir, Avi Yizhak, Bracha Rager, Ran Taube, Yacov Weinstein, Hila Naaman, Yonat Shemer Avni, Glenn F. Rall, Jacob Gopas, and Tatiana Rabinski
- Subjects
0301 basic medicine ,Cell type ,Green Fluorescent Proteins ,Induced Pluripotent Stem Cells ,Cell ,Mice, SCID ,Germ layer ,Cell Line ,Membrane Cofactor Protein ,Measles virus ,Mice ,03 medical and health sciences ,Signaling Lymphocytic Activation Molecule Family Member 1 ,Mice, Inbred NOD ,medicine ,Animals ,Humans ,Cell Lineage ,Induced pluripotent stem cell ,Receptor ,biology ,CD46 ,food and beverages ,Cell Differentiation ,Cell Biology ,biology.organism_classification ,Cell biology ,MicroRNAs ,030104 developmental biology ,medicine.anatomical_structure ,Cell culture ,Receptors, Virus ,Brief Communications ,Developmental Biology ,Biotechnology - Abstract
In this study, we found that the measles virus (MV) can infect human-induced pluripotent stem cells (hiPSCs). Wild-type MV strains generally use human signaling lymphocyte activation molecule (SLAM; CD150) as a cellular receptor, while vaccine strains such as the Edmonston strain can use both CD150 and CD46 as receptors. It is not yet known how early in the embryonal differentiation stages these receptors are expressed. We established two hiPSCs (BGU-iPSCs and EMF-iPSCs) which express CD46 and CD150. Both cell types can be infected by MV to form persistent, noncytopathic cell lines that release infectious MV particles. Following MV persistent infection, BGU-iPSCs and EMF-iPSCs remain pluripotent and can differentiate in vitro into the three germ layers. This includes cells expressing the neuronal differentiation markers: NF68 and miRNA-124. Since the MV does not integrate into the cell's genome, it can be utilized as a vehicle to systematically introduce genes into iPSC, to dissect and to define factors regulating lineage differentiation.
- Published
- 2018
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