1. Regulation of Fc gamma receptor subtype expression on a human eosinophilic leukemia cell line EoL-3: participation of cAMP and protein kinase C in the effects of interferon-gamma and phorbol ester.
- Author
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Nambu M, Watanabe H, Kim KM, Tanaka M, Mayumi M, and Mikawa H
- Subjects
- 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine, Antibodies, Monoclonal, Antigens, Differentiation classification, Bucladesine pharmacology, Calcium physiology, Dexamethasone pharmacology, Dose-Response Relationship, Drug, Flow Cytometry, Humans, Immunoglobulin G metabolism, In Vitro Techniques, Isoquinolines pharmacology, Leukemia, Eosinophilic, Acute immunology, Leukemia, Eosinophilic, Acute pathology, Piperazines pharmacology, Receptors, Fc classification, Receptors, IgG, Signal Transduction, Tumor Cells, Cultured, Antigens, Differentiation metabolism, Cyclic AMP physiology, Eosinophils immunology, Interferon-gamma pharmacology, Protein Kinase C physiology, Receptors, Fc metabolism, Tetradecanoylphorbol Acetate pharmacology
- Abstract
We investigated the effects of interferon-gamma (IFN-gamma), phorbol myristate acetate (PMA), and dibutyryl cAMP (Bt2cAMP) on Fc gamma R subtype expression on a human eosinophilic leukemia cell line, EoL-3. Unstimulated EoL-3 cells expressed Fc gamma RII as determined by monoclonal antibody (mAb) IV-3, whereas there was little or no Fc gamma RI and Fc gamma RIII expression as determined by mAbs 32.2 and 3G8, respectively. IFN-gamma induced Fc gamma RI expression, and Bt2 cAMP, which did not induce Fc gamma RI expression by itself, showed an additive effect on IFN-gamma-induced Fc gamma RI expression. Fc gamma RII expression was augmented by IFN-gamma, PMA, and Bt2 cAMP. Bt2 cAMP also showed an additive effect on IFN-gamma-augmented Fc gamma RII expression. Fc gamma RIII expression could be induced only by IFN-gamma plus Bt2 cAMP. H-7, a protein kinase C (PK-C) inhibitor, suppressed the enhancement of Fc gamma R subtype expression induced by these reagents. These results show that Fc gamma R subtype expression on EoL-3 cells is regulated differently in each subtype and that cAMP and PK-C play important roles in the regulation.
- Published
- 1991
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