1. Functional role of inflammation in the surgical injury induced vascular remodeling of male albino rats
- Author
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Xiuxia Li, Xiaoyu Song, Yong-Na Wu, Lu-Yuan Li, and Zhang Ling'en
- Subjects
0301 basic medicine ,Functional role ,Male ,Pathology ,medicine.medical_specialty ,HSP27 Heat-Shock Proteins ,Inflammation ,030204 cardiovascular system & hematology ,Biology ,Vascular Remodeling ,03 medical and health sciences ,0302 clinical medicine ,Hsp27 ,Western blot ,Heat shock protein ,medicine ,Animals ,HSP70 Heat-Shock Proteins ,Intraoperative Complications ,Close contact ,HSP47 Heat-Shock Proteins ,medicine.diagnostic_test ,NF-kappa B ,General Medicine ,Surgical Injury ,Hsp70 ,Rats ,030104 developmental biology ,Carotid Arteries ,biology.protein ,medicine.symptom ,Carotid Artery Injuries ,Vascular Surgical Procedures - Abstract
Our present study investigates the cellular and molecular inflammatory events in male albino rat arterial injury. Male albino rats were subjected to longitudinal incision and carotid artery clamping for the duration of 45 days. Heat shock protein (HSP) 27, HSP70, HSP47 and Nuclear Factor kappa B (NF-κB) expressions were determined by qPCR and Western blot method. The morphology of vessel wall alteration was studied by the light microscopy. The expression of NF-κB was found to be increased after ten days of carotid artery injury. The qPCR and Western blot analysis showed elevation in HSP47, HSP27, and HSP70 expression, ten days following the surgical injury. The neointima-formation and the media layer discontinuity were evidenced by light microscopy. The dendritic-like cells were in close contact with the lymphocytes. Our study reports that the surgical injury induces an inflammatory response through the increased NF-κB and HSPs expression.
- Published
- 2017