1. Mechanical Control of Cell Migration by the Metastasis Suppressor Tetraspanin CD82/KAI1.
- Author
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Ordas, Laura, Costa, Luca, Lozano, Anthony, Chevillard, Christopher, Calovoulos, Alexia, Kantar, Diala, Fernandez, Laurent, Chauvin, Lucie, Dosset, Patrice, Doucet, Christine, Heron-Milhavet, Lisa, Odintsova, Elena, Berditchevski, Fedor, Milhiet, Pierre-Emmanuel, and Bénistant, Christine
- Subjects
TUMOR suppressor proteins ,TETRASPANIN ,FOCAL adhesions ,BREAST cancer ,CELL migration ,MEMBRANE proteins - Abstract
The plasma membrane is a key actor of cell migration. For instance, its tension controls persistent cell migration and cell surface caveolae integrity. Then, caveolae constituents such as caveolin-1 can initiate a mechanotransduction loop that involves actin- and focal adhesion-dependent control of the mechanosensor YAP to finely tune cell migration. Tetraspanin CD82 (also named KAI-1) is an integral membrane protein and a metastasis suppressor. Its expression is lost in many cancers including breast cancer. It is a strong inhibitor of cell migration by a little-known mechanism. We demonstrated here that CD82 controls persistent 2D migration of EGF-induced single cells, stress fibers and focal adhesion sizes and dynamics. Mechanistically, we found that CD82 regulates membrane tension, cell surface caveolae abundance and YAP nuclear translocation in a caveolin-1-dependent manner. Altogether, our data show that CD82 controls 2D cell migration using membrane-driven mechanics involving caveolin and the YAP pathway. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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